伯氏疏螺旋体的补体杀伤作用。靶抗原和致敏抗体。

The complement-killing of Borrelia burgdorferi. Target antigens and sensitizing antibodies.

作者信息

Cacciapuoti B, Ciarrocchi S, Ciceroni L

机构信息

Department of Bacteriology and Medical Mycology, Istituto Superiore di Sanità, Rome, Italy.

出版信息

Zentralbl Bakteriol. 1998 Jul;288(1):121-9. doi: 10.1016/s0934-8840(98)80109-6.

DOI:10.1016/s0934-8840(98)80109-6

PMID:9728412

Abstract

It had been previously shown by the Microbial Adherence Immobilization Assay (MAIA) that Borrelia burgdorferi sensu stricto, type strain B31 was clumped, immobilized and killed in vitro by sensitizing antibodies that activated the classical complement pathway and the complement-killing of live borrelia. In the present study, the target antigens and sensitizing antibodies responsible for the complement-killing of borrelia were investigated, using MAIA as a selective identification tool. It was found that the fractions containing the 31 and 34 kDa outer surface proteins from strain B31 were the unique antigens producing sensitizing antibodies in rabbits that activated the complement-killing of B31. An anti-OspB, but not an anti-OspA, monoclonal antibody did activate the B31 complement-killing in MAIA. From these results, constraints on the effectiveness of OspB and OspA as immunogens for the prevention and control of Lyme borreliosis in humans are discussed.

摘要

先前通过微生物黏附固定化测定法（MAIA）表明，严格意义上的伯氏疏螺旋体，即模式菌株B31，在体外会因激活经典补体途径的致敏抗体而发生凝集、固定并被杀死，且活的疏螺旋体会被补体杀伤。在本研究中，以MAIA作为一种选择性鉴定工具，对负责疏螺旋体补体杀伤的靶抗原和致敏抗体进行了研究。结果发现，来自菌株B31的含有31 kDa和34 kDa外表面蛋白的组分是在兔体内产生致敏抗体的独特抗原，这些致敏抗体可激活对B31的补体杀伤作用。一种抗OspB单克隆抗体而非抗OspA单克隆抗体在MAIA中确实能激活对B31的补体杀伤作用。基于这些结果，讨论了OspB和OspA作为人类莱姆病预防和控制免疫原有效性的限制因素。