Tatari Hasan, Skiak Eyad, Destan Hakan, Ulukuş Cağnur, Ozer Erdener, Satoğlu Safa
Department of Orthopaedics and Traumatology, Dokuz Eylül University, School of Medicine, Izmir, Turkey.
Arch Phys Med Rehabil. 2004 Sep;85(9):1470-4. doi: 10.1016/j.apmr.2003.09.022.
To investigate the local effects of hylan G-F 20 on locally administered corticosteroid-induced experimental Achilles' tendonitis.
Before-after trial.
Institutional practice.
Convenience sample of 18 male Wistar white rats (weight range, 322-375 g).
After performing Achilles' degeneration with local corticosteroid injections, the rats were divided into 4 groups. The right Achilles' tendon of the rats served as the hylan injection group and the left tendon as the control group, which was injected with saline at 5-day intervals. The tendons and paratenons were excised at the end of 60 or 75 days and evaluated histopathologically and statistically.
Histopathologic changes, including staining affinity, nuclear appearance, fibrillar appearance for tendon and thickness, occurrence of fibrosis and edema, capillary changes, and inflammation for paratenon, were assessed according to a semiquantitative scoring system. The Mann-Whitney U test was used for statistical analysis, with a P value of.05 or less considered statistically significant.
Semiquantitative scoring of histopathologic changes showed that histologic appearances differed between the hylan and saline groups and between 2 hylan groups. Hylan-injected tendons and paratenons demonstrated significantly lower scores, especially after 75 days.
Hylan G-F 20 has a promising curative effect on the tendon and paratenon and can be used in Achilles' tendonitis. This finding should be supported by biomechanical and biochemical studies.
研究透明质烷G-F 20对局部注射皮质类固醇诱导的实验性跟腱炎的局部影响。
前后对照试验。
机构实践。
18只雄性Wistar大白鼠的便利样本（体重范围322 - 375克）。
通过局部注射皮质类固醇造成跟腱退变后，将大鼠分为4组。大鼠的右侧跟腱作为透明质烷注射组，左侧跟腱作为对照组，对照组每隔5天注射生理盐水。在60天或75天结束时切除肌腱和腱旁组织，并进行组织病理学评估和统计学分析。
根据半定量评分系统评估组织病理学变化，包括肌腱的染色亲和力、细胞核外观、纤维外观和厚度、纤维化和水肿的发生情况、毛细血管变化以及腱旁组织的炎症情况。采用Mann-Whitney U检验进行统计学分析，P值≤0.05被认为具有统计学意义。
组织病理学变化的半定量评分显示，透明质烷组和生理盐水组之间以及两个透明质烷组之间的组织学表现存在差异。注射透明质烷的肌腱和腱旁组织得分显著更低，尤其是在75天后。
透明质烷G-F 20对肌腱和腱旁组织具有良好的治疗效果，可用于跟腱炎。这一发现应得到生物力学和生化研究的支持。
