Sullo A, Maffulli N, Capasso G, Testa V
Department of Sports Physiopathology, University of Napoli, Napoli, Italy.
J Orthop Sci. 2001;6(4):349-57. doi: 10.1007/s007760100031.
We studied the effects of peritendinous Achilles tendon injections of prostaglandin E1 (PGE1) on the Achilles tendon of rats. Five groups of Sprague-Dawley rats (n = 24 each) were studied. Groups 1 to 4 received weekly peritendinous injections. In group 1, one side was injected with 800 ng of PGE1 in 0.5 ml of 0.9% NaCl and the contralateral side was injected with 0.5 ml of 0.9% NaCl. In group 2, one side was injected with 800 ng of PGE1. In group 3, one side was injected with 0.5 ml of 0.9% NaCl. In group 4, a syringe needle was inserted in the peritenon unilaterally, but no substances were administered. In groups 2, 3, and 4, the contralateral tendon was used as the control. In group 5, treatment was not administered. Eight rats in each group were killed at each time point, after 7, 21, and 35 days of treatment. On day 7, values for average water content and average wet weight of the tendons treated with PGE1 were significantly higher than those in the control tendons (analysis of variance [ANOVA]; P = 0.02), with a histological picture of acute inflammation. On day 21, approximately half of the PGE1-treated tendons showed fibrosis of the paratenon, with adhesions and intra-tendinous degeneration, with the other half still showing a picture of acute inflammation. On day 35, all of the PGE1-treated tendons showed fibrosis of the paratenon, with adhesions and intra-tendinous degeneration. At all time points, there was no evidence of pathology in the tendons that had not received PGE1. Sham peritendinous injections and injections of normal saline did not produce inflammation in the Achilles tendons. Initially, local administration of PGE1 produced acute inflammation of the tendon and its surrounding tissues. Prolonged PGE1 administration produced peri- and intra-tendinous degeneration, providing a cheap, reproducible model of Achilles tendinopathy, which would allow studies of the effects of conservative and surgical management of the condition.
我们研究了前列腺素E1（PGE1）经皮内注射至大鼠跟腱对跟腱的影响。研究了五组Sprague-Dawley大鼠（每组n = 24）。第1至4组每周接受经皮内注射。在第1组中，一侧注射800 ng PGE1于0.5 ml 0.9%氯化钠溶液中，对侧注射0.5 ml 0.9%氯化钠溶液。在第2组中，一侧注射800 ng PGE1。在第3组中，一侧注射0.5 ml 0.9%氯化钠溶液。在第4组中，将注射器针头单侧插入腱周，但不给予任何物质。在第2、3和4组中，对侧肌腱用作对照。在第5组中，不给予治疗。在治疗7、21和35天后的每个时间点，每组处死8只大鼠。在第7天，用PGE1治疗的肌腱的平均含水量和平均湿重值显著高于对照肌腱（方差分析[ANOVA]；P = 0.02），组织学表现为急性炎症。在第21天，约一半用PGE1治疗的肌腱显示腱旁组织纤维化，伴有粘连和肌腱内变性，另一半仍显示急性炎症表现。在第35天，所有用PGE1治疗的肌腱均显示腱旁组织纤维化，伴有粘连和肌腱内变性。在所有时间点，未接受PGE1的肌腱均无病理证据。假经皮内注射和注射生理盐水未在跟腱中产生炎症。最初，局部给予PGE1会导致肌腱及其周围组织的急性炎症。长期给予PGE1会导致腱周和肌腱内变性，提供了一种廉价、可重复的跟腱病模型，这将有助于研究该疾病保守和手术治疗的效果。
