瑞香狼毒提取物的体外抗肿瘤活性——系统溶剂萃取法研究
[The antitumor activities of the extracts from Stellera chamaejasmel L. in vitro by means of systematic solvent extraction].
作者信息
Wang Bin, Wang Rui, Jia Zhengping, Fan Junjie, Ma Jun, Zhang Ruxue
机构信息
School of Life Science, Lanzhou University, Lanzhou 730000.
出版信息
Zhong Yao Cai. 2004 May;27(5):355-7.
OBJECTIVE
To screen the antitumor extract from Stellera chamaejasmel L..
METHODS
The means of systematic solvent extraction was used to separate the 95% ethanol extract. The antitumor activities of the different extracts in vitro were analyzed with MTT assay.
RESULTS
The petroleum ether extract and the chloroform extract strongly inhibited cell proliferation of human cancer cell lines such as leukemia HL-60, stomach cancer SGC-7901 and hepatocarcinoma BEL-7402, and showed significant dose-dependent response while the n-butanol extract and the methanol extract exhibited low antitumor activities in vitro. The petroleum ether extract was most active.
CONCLUSION
The petroleum ether extract is the principal antitumor extract from Stellera chamaejasmel L..
目的
筛选瑞香狼毒的抗肿瘤提取物。
方法
采用系统溶剂萃取法分离95%乙醇提取物。用MTT法分析不同提取物的体外抗肿瘤活性。
结果
石油醚提取物和氯仿提取物强烈抑制人癌细胞系如白血病HL-60、胃癌SGC-7901和肝癌BEL-7402的细胞增殖，并呈现显著的剂量依赖性反应，而正丁醇提取物和甲醇提取物在体外表现出较低的抗肿瘤活性。石油醚提取物活性最强。
结论
石油醚提取物是瑞香狼毒的主要抗肿瘤提取物。
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