补骨脂活性成分的提取分离及其体外抗肿瘤作用研究
[Studies on extraction and isolation of active constituents from Psoralen corylifolia L. and the antitumor effect of the constituents in vitro].
作者信息
Guo Jiangning, Wu Hou, Weng Xinchu, Yan Jianhua, Bi Kaishun
机构信息
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016.
出版信息
Zhong Yao Cai. 2003 Mar;26(3):185-7.
OBJECTIVE
To study the chemical constituents from Psoralea corylifolia L. and their antitumor activities.
METHODS
The constituents were isolated on silica gel column chromatography and identified by physicochemical properties and spectroscopic analysis. Morphological and MTT assays in vitro were used to explore the inhibiting effect of the active compounds on the proliferation of BGC-823 cancer cell.
RESULTS
Two compounds were isolated from its chloroform extract and identified as psoralen (I) and isopsoralen (II). IC50 of psoralen and isoporalen were 5.82 micrograms/ml and 148.8 micrograms/ml respectively.
CONCLUSION
Compound I and II having antitumor activity against BGC-823 cancer cell were obtained from this plant with the yields of 0.048% and 0.11%.
目的
研究补骨脂的化学成分及其抗肿瘤活性。
方法
采用硅胶柱色谱法分离化合物，并通过理化性质和光谱分析进行鉴定。利用形态学和体外MTT法探讨活性化合物对BGC - 823癌细胞增殖的抑制作用。
结果
从其氯仿提取物中分离得到两种化合物，分别鉴定为补骨脂素（I）和异补骨脂素（II）。补骨脂素和异补骨脂素的IC50分别为5.82微克/毫升和148.8微克/毫升。
结论
从该植物中获得了对BGC - 823癌细胞具有抗肿瘤活性的化合物I和II，产率分别为0.048%和0.11%。
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