乌干达镰状细胞病患儿唾液和血沉棕黄层样本中卡波西肉瘤相关疱疹病毒DNA的检测

Detection of kaposi sarcoma-associated herpesvirus DNA in saliva and buffy-coat samples from children with sickle cell disease in Uganda.

作者信息

Mbulaiteye Sam M, Pfeiffer Ruth M, Engels Eric A, Marshall Vickie, Bakaki Paul M, Owor Anchilla M, Ndugwa Christopher M, Katongole-Mbidde Edward, Goedert James J, Biggar Robert J, Whitby Denise

机构信息

Division of Cancer Epidemiology and Genetics, National Cancer Institute, Department of Health and Human Services, Rockville, Maryland, USA.

出版信息

J Infect Dis. 2004 Oct 15;190(8):1382-6. doi: 10.1086/424489. Epub 2004 Sep 10.

DOI:10.1086/424489

PMID:15378429

Abstract

Among 233 children, Kaposi sarcoma-associated herpesvirus (KSHV) DNA was detected in 43% of children seropositive for both K8.1 and orf73, in 29% of children seropositive for K8.1 only, in 14% of children seropositive for orf73 only, and in 7% of children seronegative for both K8.1 and orf73; among 228 mothers, KSHV DNA was detected in 27%, 25%, 4%, and 1%, respectively. KSHV DNA was detected more frequently and at higher levels in saliva than in buffy-coat samples and in children than in mothers. In both children and mothers, detection in saliva was associated with detection in peripheral blood. Detection was associated with K8.1 seropositivity, younger age, and high household density, indicating the importance of in-household person-to-person transmission, likely via saliva.

摘要

在233名儿童中，K8.1和orf73均呈血清阳性的儿童中，43%检测到卡波西肉瘤相关疱疹病毒（KSHV）DNA；仅K8.1呈血清阳性的儿童中，29%检测到该病毒DNA；仅orf73呈血清阳性的儿童中，14%检测到该病毒DNA；K8.1和orf73均呈血清阴性的儿童中，7%检测到该病毒DNA。在228名母亲中，上述比例分别为27%、25%、4%和1%。与血沉棕黄层样本相比，唾液中KSHV DNA的检测频率更高、水平更高；与母亲相比，儿童中KSHV DNA的检测频率更高、水平更高。在儿童和母亲中，唾液检测结果均与外周血检测结果相关。检测结果与K8.1血清阳性、年龄较小和家庭人口密度高有关，表明家庭内人际传播（可能通过唾液）的重要性。