Mbulaiteye Sam M, Walters Michael, Engels Eric A, Bakaki Paul M, Ndugwa Christopher M, Owor Anchilla M, Goedert James J, Whitby Denise, Biggar Robert J
Division of Cancer Epidemiology and Genetics, National Cancer Institute, Department of Health and Human Services, Rockville, Maryland, USA.
J Infect Dis. 2006 Feb 1;193(3):422-6. doi: 10.1086/499277. Epub 2005 Dec 22.
In Africa, Epstein-Barr virus (EBV) is associated with Burkitt lymphoma. We measured levels of EBV DNA in saliva and buffy coats from 233 asymptomatic Ugandan children with sickle cell disease and their mothers by quantitative real-time polymerase chain reaction. EBV DNA was detected in saliva from 90% of the children (median [interquartile range [IQR]] level, 5.2 [4.2-6.0] log(10) copies/mL of saliva) and 79% of the mothers (median [IQR] level, 4.8 [3.7-5.6] log(10) copies/mL of saliva) (P < .001). EBV DNA was detected in buffy coats from 86% of the children (median [IQR] level, 2.5 [2.2-2.9] log(10) copies/1 x 10(6) peripheral white blood cells [PWBCs]) and 72% of the mothers (median [IQR] level, 2.7 [2.4-3.1] log(10) copies/1 x 10(6) PWBCs) (P = .24). Detection of EBV DNA in saliva was positively correlated with detection in buffy coats. EBV DNA was detected more frequently in saliva and buffy coats than was human herpesvirus 8 DNA. Our results indicate that EBV infection persists, with virus readily detectable in saliva and buffy coats from persons without apparent symptoms in Africa.
在非洲，爱泼斯坦-巴尔病毒（EBV）与伯基特淋巴瘤有关。我们通过定量实时聚合酶链反应测量了233名无症状的乌干达镰状细胞病患儿及其母亲唾液和血沉棕黄层中的EBV DNA水平。在90%的儿童唾液中检测到EBV DNA（中位数[四分位间距[IQR]]水平，5.2[4.2 - 6.0] log₁₀拷贝/毫升唾液），79%的母亲唾液中检测到EBV DNA（中位数[IQR]水平，4.8[3.7 - 5.6] log₁₀拷贝/毫升唾液）（P <.001）。在86%的儿童血沉棕黄层中检测到EBV DNA（中位数[IQR]水平，2.5[2.2 - 2.9] log₁₀拷贝/1×10⁶外周血白细胞[PWBCs]），72%的母亲血沉棕黄层中检测到EBV DNA（中位数[IQR]水平，2.7[2.4 - 3.1] log₁₀拷贝/1×10⁶PWBCs）（P = 0.24）。唾液中EBV DNA的检测与血沉棕黄层中的检测呈正相关。唾液和血沉棕黄层中EBV DNA的检测频率高于人类疱疹病毒8 DNA。我们的结果表明，EBV感染持续存在，在非洲无明显症状者的唾液和血沉棕黄层中很容易检测到该病毒。
