Zhao Li, Chang Jiang
Biomaterials and Tissue Engineering Research Center, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai 200050, P.R. China.
J Mater Sci Mater Med. 2004 May;15(5):625-9. doi: 10.1023/b:jmsm.0000026103.44687.d0.
Chitosan/wollastonite composite scaffolds were prepared by a thermally induced phase separation method. The microstructure, mechanical performance and in vitro bioactivity of the composite scaffolds were investigated. The composite scaffolds were macroporous and wollastonite particles were dispersed uniformly on the surface of the pore walls. Scanning electron microscope images of the composite scaffolds demonstrated that the scaffolds had interconnected pores with diameters from 60 to 200 microm. Both the pore size and structure were affected by freezing temperature. The mechanical performance of the composite scaffolds was improved compared to that of pure chitosan scaffolds. The in vitro bioactivity of the scaffolds was evaluated by soaking samples in simulated body fluid and the apatite layer was observed on the surface of the pore walls of the composite scaffolds. Our results suggest that the incorporation of wollastonite into chitosan could enhance both the mechanical strength and the in vitro bioactivity of the resultant composite. The macroporous chitosan/wollastonite scaffolds may be a potential candidate for application in tissue engineering.
采用热致相分离法制备了壳聚糖/硅灰石复合支架。研究了复合支架的微观结构、力学性能和体外生物活性。复合支架为大孔结构，硅灰石颗粒均匀分散在孔壁表面。复合支架的扫描电子显微镜图像表明，支架具有相互连通的孔隙，孔径为60至200微米。孔径和结构均受冷冻温度的影响。与纯壳聚糖支架相比，复合支架的力学性能有所提高。通过将样品浸泡在模拟体液中来评估支架的体外生物活性，在复合支架孔壁表面观察到了磷灰石层。我们的结果表明，将硅灰石掺入壳聚糖中可以提高所得复合材料的机械强度和体外生物活性。大孔壳聚糖/硅灰石支架可能是组织工程应用的潜在候选材料。
