喹诺酮耐药决定区gyrA、gyrB、parC和parE内突变的发生率以及与耐喹诺酮肠炎沙门氏菌抗生素耐药性的关联
Prevalence of mutations within the quinolone resistance-determining region of gyrA, gyrB, parC, and parE and association with antibiotic resistance in quinolone-resistant Salmonella enterica.
作者信息
Eaves Deborah J, Randall Luke, Gray Douglas T, Buckley Antony, Woodward Martin J, White Allan P, Piddock Laura J V
机构信息
Antimicrobial Agents Research Group, Division of Immunity and Infection, University of Birmingham, Birmingham B15 2TT, United Kingdom.
出版信息
Antimicrob Agents Chemother. 2004 Oct;48(10):4012-5. doi: 10.1128/AAC.48.10.4012-4015.2004.
Abstract
Salmonella enterica isolates (n = 182) were examined for mutations in the quinolone resistance-determining region of gyrA, gyrB, parC, and parE. The frequency, location, and type of GyrA substitution varied with the serovar. Mutations were found in parC that encoded Thr57-Ser, Thr66-Ile, and Ser80-Arg substitutions. Mutations in the gyrB quinolone resistance-determining region were located at codon Tyr420-Cys or Arg437-Leu. Novel mutations were also found in parE encoding Glu453-Gly, His461-Tyr, Ala498-Thr, Val512-Gly, and Ser518-Cys. Although it is counterintuitive, isolates with a mutation in both gyrA and parC were more susceptible to ciprofloxacin than were isolates with a mutation in gyrA alone.
摘要
对182株肠炎沙门氏菌分离株进行了gyrA、gyrB、parC和parE喹诺酮耐药决定区的突变检测。GyrA替代的频率、位置和类型因血清型而异。在parC中发现了编码Thr57-Ser、Thr66-Ile和Ser80-Arg替代的突变。gyrB喹诺酮耐药决定区的突变位于密码子Tyr420-Cys或Arg437-Leu。在parE中也发现了新的突变，编码Glu453-Gly、His461-Tyr、Ala498-Thr、Val512-Gly和Ser518-Cys。尽管有悖常理，但与仅gyrA发生突变的分离株相比，gyrA和parC均发生突变的分离株对环丙沙星更敏感。
相似文献
1
Prevalence of mutations within the quinolone resistance-determining region of gyrA, gyrB, parC, and parE and association with antibiotic resistance in quinolone-resistant Salmonella enterica.
Antimicrob Agents Chemother. 2004 Oct;48(10):4012-5. doi: 10.1128/AAC.48.10.4012-4015.2004.
2
Mutations in the gyrB, parC, and parE genes of quinolone-resistant isolates and mutants of Edwardsiella tarda.
J Microbiol Biotechnol. 2010 Dec;20(12):1735-43.
3
Prevalence of plasmid-mediated quinolone resistance and mutations in the gyrase and topoisomerase IV genes in Salmonella isolated from 12 tertiary-care hospitals in Korea.
Microb Drug Resist. 2011 Dec;17(4):551-7. doi: 10.1089/mdr.2011.0095. Epub 2011 Aug 10.
4
Detection of mutations in Salmonella enterica gyrA, gyrB, parC and parE genes by denaturing high performance liquid chromatography (DHPLC) using standard HPLC instrumentation.
J Antimicrob Chemother. 2005 Oct;56(4):619-23. doi: 10.1093/jac/dki293. Epub 2005 Sep 1.
5
Contribution of mutations in DNA gyrase and topoisomerase IV genes to ciprofloxacin resistance in Escherichia coli clinical isolates.
Int J Antimicrob Agents. 2011 Mar;37(3):253-5. doi: 10.1016/j.ijantimicag.2010.11.022. Epub 2011 Jan 13.
6
[Fluoroquinolone resistance mutations in topoisomerase genes of Salmonella typhimurium isolates].
Wei Sheng Yan Jiu. 2004 Sep;33(5):591-4.
7
Hotspots sequences of gyrA, gyrB, parC, and parE genes encoded for fluoroquinolones resistance from local Salmonella Typhi strains in Jakarta.
BMC Microbiol. 2022 Oct 18;22(1):250. doi: 10.1186/s12866-022-02666-z.
8
Molecular characterization of ciprofloxacin-resistant Salmonella enterica serovar Typhi and Paratyphi A causing enteric fever in India.
J Antimicrob Chemother. 2006 Dec;58(6):1139-44. doi: 10.1093/jac/dkl391. Epub 2006 Oct 28.
9
High-level resistance to fluoroquinolones linked to mutations in gyrA, parC, and parE in Salmonella enterica serovar Schwarzengrund isolates from humans in Taiwan.
Antimicrob Agents Chemother. 2005 Feb;49(2):862-3. doi: 10.1128/AAC.49.2.862-863.2005.
10
Mutations in topoisomerase genes of fluoroquinolone-resistant salmonellae in Hong Kong.
Antimicrob Agents Chemother. 2003 Nov;47(11):3567-73. doi: 10.1128/AAC.47.11.3567-3573.2003.
引用本文的文献
1
Unveiling the Genetic Diversity and Antimicrobial Resistance Profiles of Salmonella Population From 2016 to 2020 in Thai Canal Water.
Environ Microbiol Rep. 2025 Aug;17(4):e70160. doi: 10.1111/1758-2229.70160.
2
The diverse phenotypic and mutational landscape induced by fluoroquinolone treatment.
mSystems. 2025 Aug 19;10(8):e0071325. doi: 10.1128/msystems.00713-25. Epub 2025 Jul 31.
3
Microbial Food Safety and Antimicrobial Resistance in Foods: A Dual Threat to Public Health.
Microorganisms. 2025 Jul 6;13(7):1592. doi: 10.3390/microorganisms13071592.
4
Herbs impact on poultry health and antimicrobial resistance: a scoping review with one health perspective.
BMC Vet Res. 2025 Jul 29;21(1):499. doi: 10.1186/s12917-025-04760-6.
5
First characterization of the resistome, virulome and genomic diversity of Salmonella enterica serovar Inganda: a rare, clinically-related and drug susceptible serovar.
Curr Genet. 2025 May 31;71(1):13. doi: 10.1007/s00294-025-01317-w.
6
Unveiling the Genomic Landscape of Understudied Salmonella enterica Serovars from Poultry and Human: Implications for Food Safety.
Curr Microbiol. 2025 May 6;82(6):279. doi: 10.1007/s00284-025-04234-w.
7
Antimicrobial Resistance and Genomic Characterization of Isolated from Pigeons in China.
Transbound Emerg Dis. 2024 May 6;2024:3315678. doi: 10.1155/2024/3315678. eCollection 2024.
8
Tracing the evolution: the rise of Thompson co-resistant to clinically important antibiotics in China, 1997-2020.
mSystems. 2025 Mar 18;10(3):e0101824. doi: 10.1128/msystems.01018-24. Epub 2025 Feb 12.
9
Computational identification of novel natural inhibitors against triple mutant DNA gyrase A in fluoroquinolone-resistant Typhimurium.
Biochem Biophys Rep. 2025 Jan 8;41:101901. doi: 10.1016/j.bbrep.2024.101901. eCollection 2025 Mar.
10
Clonal Spread and Genetic Mechanisms Underpinning Ciprofloxacin Resistance in .
Foods. 2025 Jan 16;14(2):289. doi: 10.3390/foods14020289.
本文引用的文献
1
Mutations in topoisomerase genes of fluoroquinolone-resistant salmonellae in Hong Kong.
Antimicrob Agents Chemother. 2003 Nov;47(11):3567-73. doi: 10.1128/AAC.47.11.3567-3573.2003.
2
European harmonization of MIC breakpoints for antimicrobial susceptibility testing of bacteria.
J Antimicrob Chemother. 2003 Aug;52(2):145-8. doi: 10.1093/jac/dkg312. Epub 2003 Jul 1.
3
Multiple resistance mechanisms in fluoroquinolone-resistant Salmonella isolates from Germany.
Antimicrob Agents Chemother. 2003 Jun;47(6):2059. doi: 10.1128/AAC.47.6.2059.2003.
4
Topoisomerase IV mutations in quinolone-resistant salmonellae selected in vitro.
Microb Drug Resist. 2003 Spring;9(1):25-32. doi: 10.1089/107662903764736319.
5
Is it time to change fluoroquinolone breakpoints for Salmonella spp.?
Antimicrob Agents Chemother. 2003 Feb;47(2):827-9. doi: 10.1128/AAC.47.2.827-829.2003.
6
Detection of gyrA mutations in quinolone-resistant Salmonella enterica by denaturing high-performance liquid chromatography.
J Clin Microbiol. 2002 Nov;40(11):4121-5. doi: 10.1128/JCM.40.11.4121-4125.2002.
7
Antimicrobial drug resistance in Salmonella: problems and perspectives in food- and water-borne infections.
FEMS Microbiol Rev. 2002 Jun;26(2):141-8. doi: 10.1111/j.1574-6976.2002.tb00606.x.
8
Fluoroquinolone resistance in Salmonella serovars isolated from humans and food animals.
FEMS Microbiol Rev. 2002 Mar;26(1):3-16. doi: 10.1111/j.1574-6976.2002.tb00596.x.
9
Determination of minimum inhibitory concentrations.
J Antimicrob Chemother. 2001 Jul;48 Suppl 1:5-16. doi: 10.1093/jac/48.suppl_1.5.
10
Mechanisms of fluoroquinolone resistance: an update 1994-1998.
Drugs. 1999;58 Suppl 2:11-8. doi: 10.2165/00003495-199958002-00003.
Zotero 插件