Benevides Valdinete P, Saraiva Mauro M S, Ferreira Viviane A, Funnicelli Michelli Inácio G, Rodrigues Alves Lucas B, Almeida Adriana M, Christensen Henrik, Olsen John E, Berchieri Junior Angelo
Postgraduate Program in Agricultural Microbiology, School of Agricultural and Veterinary Sciences, São Paulo State University (UNESP), Jaboticabal, 14884-900, Brazil.
School of Agricultural and Veterinary Sciences, São Paulo State University (UNESP), Jaboticabal, SP, 14884-900, Brazil.
Curr Microbiol. 2025 May 6;82(6):279. doi: 10.1007/s00284-025-04234-w.
Despite the bacteria of the genus Salmonella are pathogens of zoonotic importance, the factors associated with some serovars genetic diversity remain unclear. We investigated genotypic profiles of antimicrobial resistance, plasmid replicons, and virulence factors in 301 S. enterica genomes from human and animal sources, supplemented by ten sequenced genomes from fecal samples of laying hens in Brazil. Many antimicrobial resistance genes have been detected across various Salmonella serovars; with a limited number of unique resistance genes predicted in poultry isolates compared to human isolates. Specifically, among the 52 antimicrobial resistance genes identified, 48% were shared between poultry and human isolates, while 21.1% were exclusive to poultry isolates and 30.7% were exclusive to human isolates. Chromosomal mutations in the gyrA and parC genes were also predicted. To the best of our knowledge, this is the first work to report S. Braenderup carrying the SPI-10. SGI-1 was detected in a few isolates of S. Schwarzengrund from poultry and the CS54 island was solely noticed in genomes referring to the serovars S. Saintpaul and S. Braenderup. Among the serovars analyzed, S. Saintpaul showed the lowest plasmid diversity. A total of 161 (161/271) virulence genes were common to all serovars, the remaining genes were exclusively identified within specific serovars, revealing a distinct distribution pattern within the S. enterica population. Overall, our study brings to light the genetic potential of Salmonella serovars frequently neglected in poultry production, which threatens public health, particularly due to multidrug-resistant profiles against active principles used to treat human infections.
尽管沙门氏菌属细菌是具有人畜共患病重要性的病原体，但与某些血清型遗传多样性相关的因素仍不清楚。我们调查了来自人类和动物源的301株肠炎沙门氏菌基因组中的抗菌药物耐药性、质粒复制子和毒力因子的基因型谱，并补充了来自巴西蛋鸡粪便样本的10个测序基因组。在各种沙门氏菌血清型中检测到了许多抗菌药物耐药基因；与人类分离株相比，家禽分离株中预测的独特耐药基因数量有限。具体而言，在鉴定出的52个抗菌药物耐药基因中，48%在家禽和人类分离株之间共享，而21.1%是家禽分离株所特有的，30.7%是人类分离株所特有的。还预测了gyrA和parC基因的染色体突变。据我们所知，这是首次报道携带SPI-10的布伦德鲁普沙门氏菌。在来自家禽的少数施瓦岑格伦德沙门氏菌分离株中检测到了SGI-1，而CS54岛仅在圣保罗沙门氏菌和布伦德鲁普沙门氏菌血清型的基因组中被发现。在所分析的血清型中，圣保罗沙门氏菌的质粒多样性最低。共有161个（161/271）毒力基因在所有血清型中是共同的，其余基因仅在特定血清型中被鉴定出来，揭示了肠炎沙门氏菌群体内独特的分布模式。总体而言，我们的研究揭示了家禽生产中经常被忽视的沙门氏菌血清型的遗传潜力，这对公共卫生构成威胁，特别是由于对用于治疗人类感染的活性成分具有多重耐药性。
