Grinstead D A, Barefoot S F
Department of Food Science, Clemson University, South Carolina 29634-0371.
Appl Environ Microbiol. 1992 Jan;58(1):215-20. doi: 10.1128/aem.58.1.215-220.1992.
The genus Propionibacterium includes cutaneous species typically found on human skin and the dairy or classical species (Propionibacterium freudenreichii, P. jensenii, P. thoenii, and P. acidipropionici) used industrially for the production of Swiss cheese and propionic acid. Grinstead (1989, M.S. thesis, Iowa State University, Ames) has previously observed that some dairy propionibacteria inhibit other species in the classical grouping. We further investigated the inhibitor(s) produced by P. jensenii P126 (ATCC 4872). An antagonist(s) from anaerobic agar cultures of P126 strongly inhibited two closely related strains of propionibacteria, P. acidipropionici P5 and P. jensenii P54, and Lactobacillus bulgaricus NCDO 1489, Lactobacillus delbrueckii subsp. lactis ATCC 4797, Lactococcus cremoris NCDO 799, and Lactococcus lactis subsp. lactis C2. The inhibitor, designated jenseniin G, was active at pH 7.0; inactivated by treatment with pronase E, proteinase K, and type 14 protease; insensitive to catalase; and stable to freezing, cold storage (4 degrees C, 3 days), and heat (100 degrees C, 15 min). Classification of the inhibitor as a bacteriocin is supported by its proteinaceous nature and its bactericidal activity against L. delbrueckii subsp. lactis ATCC 4797. The lack of detectable plasmids suggests a chromosomal location for the determinant(s) of jenseniin G.
丙酸杆菌属包括通常存在于人体皮肤上的皮肤种类以及用于瑞士奶酪和丙酸工业生产的乳制品或经典种类（费氏丙酸杆菌、詹氏丙酸杆菌、索氏丙酸杆菌和丙酸丙酸杆菌）。格林斯特德（1989年，硕士论文，爱荷华州立大学，艾姆斯）此前观察到一些乳制品丙酸杆菌会抑制经典分类中的其他种类。我们进一步研究了詹氏丙酸杆菌P126（ATCC 4872）产生的抑制剂。来自P126厌氧琼脂培养物的一种拮抗剂强烈抑制了两种密切相关的丙酸杆菌菌株，即丙酸丙酸杆菌P5和詹氏丙酸杆菌P54，以及保加利亚乳杆菌NCDO 1489、德氏乳杆菌保加利亚亚种ATCC 4797、乳脂乳球菌NCDO 799和乳酸乳球菌乳酸亚种C2。这种抑制剂被命名为詹氏菌素G，在pH 7.0时具有活性；经链霉蛋白酶E、蛋白酶K和14型蛋白酶处理后失活；对过氧化氢酶不敏感；并且对冷冻、冷藏（4℃，3天）和加热（100℃，15分钟）稳定。该抑制剂的蛋白质性质及其对德氏乳杆菌保加利亚亚种ATCC 4797的杀菌活性支持将其归类为细菌素。未检测到质粒表明詹氏菌素G的决定簇位于染色体上。
