Britz T J, Riedel K H
Department of Microbiology and Biochemistry, University of the Orange Free State, Bloemfontein, South Africa.
Int J Food Microbiol. 1994 Jun;22(4):257-67. doi: 10.1016/0168-1605(94)90177-5.
Thirteen 'classical' Propionibacterium strains, isolated from Leerdammer cheese samples, using three different media were characterized phenotypically. The phenotypic data of 74 tests, conducted on 27 propionibacteria, including four type, 10 reference strains and the 13 cheese isolates were analysed by numerical taxonomical techniques, using the simple matching coefficient and single linkage cluster analysis. All the strains were grouped in four major clusters, with a final linkage at the 81% S-level. The clusters were equated with the 'classical' P. acidipropionici, P. freudenreichii, P. jensenii and P. thoenii species. The species were identified by relating them to specific type strains and by comparison of phenotypic characteristics. Differential characteristics of each cluster were determined. Strains of P. acidipropionici, P. freudenreichii and P. jensenii, but no P. thoenii strains were isolated from the Leerdammer cheese samples. No 'cutaneous' propionibacteria were isolated. The largest cluster, representing 46% of the cheese isolates was equated with P. jensenii. Various red/brown pigmented strains, which could be identified as the old 'P. rubrum' species were isolated from the cheese. These strains were, however, phenotypically identified as P. jensenii and also grouped in the P. jensenii cluster.
从莱尔丹姆奶酪样品中分离出13株“经典”丙酸杆菌菌株，使用三种不同培养基对其进行表型特征分析。对包括4株模式菌株、10株参考菌株和13株奶酪分离株在内的27株丙酸杆菌进行了74项测试，通过数值分类技术，采用简单匹配系数和单链聚类分析对表型数据进行了分析。所有菌株被分为四个主要聚类群，在81%的相似度水平上有最终聚类。这些聚类群等同于“经典”的丙酸丙酸杆菌、费氏丙酸杆菌、詹氏丙酸杆菌和托氏丙酸杆菌物种。通过将它们与特定模式菌株相关联并比较表型特征来鉴定这些物种。确定了每个聚类群的鉴别特征。从莱尔丹姆奶酪样品中分离出了丙酸丙酸杆菌、费氏丙酸杆菌和詹氏丙酸杆菌的菌株，但未分离出托氏丙酸杆菌菌株。未分离出“皮肤”丙酸杆菌。最大的聚类群占奶酪分离株的46%，等同于詹氏丙酸杆菌。从奶酪中分离出了各种红色/棕色色素菌株，这些菌株可被鉴定为旧的“红色丙酸杆菌”物种。然而，这些菌株在表型上被鉴定为詹氏丙酸杆菌，并且也归入詹氏丙酸杆菌聚类群。
