参与迈耶氏钩端螺旋体蛋氨酸生物合成途径的高丝氨酸O-乙酰转移酶不受反馈抑制。
Homoserine O-acetyltransferase, involved in the Leptospira meyeri methionine biosynthetic pathway, is not feedback inhibited.
作者信息
Bourhy P, Martel A, Margarita D, Saint Girons I, Belfaiza J
机构信息
Unité de Bacteriologie Moleculaire et Mf1edicale, Institut Pasteur, Paris, France.
出版信息
J Bacteriol. 1997 Jul;179(13):4396-8. doi: 10.1128/jb.179.13.4396-4398.1997.
Abstract
The Leptospira meyeri serovar semaranga metX gene was identified by complementation of an Escherichia coli metA mutant, i.e., devoid of homoserine O-succinyltransferase. However, the MetX protein exhibited a homoserine O-acetyltransferase activity in agreement with its similarity to homoserine O-acetyltransferases. Reverse transcription-PCR analysis demonstrated that metX is the second gene of an operon.
摘要
通过对缺乏高丝氨酸O-琥珀酰转移酶的大肠杆菌metA突变体进行互补，鉴定出了迈耶氏钩端螺旋体塞马朗血清型的metX基因。然而，MetX蛋白表现出高丝氨酸O-乙酰转移酶活性，这与它与高丝氨酸O-乙酰转移酶的相似性相符。逆转录-聚合酶链反应分析表明，metX是一个操纵子的第二个基因。
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