• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

参与迈耶氏钩端螺旋体蛋氨酸生物合成途径的高丝氨酸O-乙酰转移酶不受反馈抑制。

Homoserine O-acetyltransferase, involved in the Leptospira meyeri methionine biosynthetic pathway, is not feedback inhibited.

作者信息

Bourhy P, Martel A, Margarita D, Saint Girons I, Belfaiza J

机构信息

Unité de Bacteriologie Moleculaire et Mf1edicale, Institut Pasteur, Paris, France.

出版信息

J Bacteriol. 1997 Jul;179(13):4396-8. doi: 10.1128/jb.179.13.4396-4398.1997.

DOI:10.1128/jb.179.13.4396-4398.1997
PMID:9209059
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC179265/
Abstract

The Leptospira meyeri serovar semaranga metX gene was identified by complementation of an Escherichia coli metA mutant, i.e., devoid of homoserine O-succinyltransferase. However, the MetX protein exhibited a homoserine O-acetyltransferase activity in agreement with its similarity to homoserine O-acetyltransferases. Reverse transcription-PCR analysis demonstrated that metX is the second gene of an operon.

摘要

通过对缺乏高丝氨酸O-琥珀酰转移酶的大肠杆菌metA突变体进行互补，鉴定出了迈耶氏钩端螺旋体塞马朗血清型的metX基因。然而，MetX蛋白表现出高丝氨酸O-乙酰转移酶活性，这与它与高丝氨酸O-乙酰转移酶的相似性相符。逆转录-聚合酶链反应分析表明，metX是一个操纵子的第二个基因。

相似文献

1
Homoserine O-acetyltransferase, involved in the Leptospira meyeri methionine biosynthetic pathway, is not feedback inhibited.参与迈耶氏钩端螺旋体蛋氨酸生物合成途径的高丝氨酸O-乙酰转移酶不受反馈抑制。
J Bacteriol. 1997 Jul;179(13):4396-8. doi: 10.1128/jb.179.13.4396-4398.1997.
2
Genetic evidence for the existence of two pathways for the biosynthesis of methionine in the Leptospira spp.钩端螺旋体属中甲硫氨酸生物合成存在两条途径的遗传学证据
FEMS Microbiol Lett. 2003 Aug 29;225(2):257-62. doi: 10.1016/S0378-1097(03)00529-9.
3
Direct sulfhydrylation for methionine biosynthesis in Leptospira meyeri.迈氏钩端螺旋体中甲硫氨酸生物合成的直接巯基化作用
J Bacteriol. 1998 Jan;180(2):250-5. doi: 10.1128/JB.180.2.250-255.1998.
4
Molecular characterization and sequence of a methionine biosynthetic locus from Pseudomonas syringae.丁香假单胞菌蛋氨酸生物合成基因座的分子特征与序列分析
J Bacteriol. 1998 Sep;180(17):4497-507. doi: 10.1128/JB.180.17.4497-4507.1998.
5
Isolation and analysis of metA, a methionine biosynthetic gene encoding homoserine acetyltransferase in corynebacterium glutamicum.谷氨酸棒杆菌中编码高丝氨酸乙酰转移酶的甲硫氨酸生物合成基因metA的分离与分析。
Mol Cells. 1998 Jun 30;8(3):286-94.
6
Regulatory region of the metA gene of Escherichia coli K-12.大肠杆菌K-12 metA基因的调控区域。
J Bacteriol. 1984 Dec;160(3):1158-62. doi: 10.1128/jb.160.3.1158-1162.1984.
7
Key amino acid residues in homoserine-acetyltransferase from M. tuberculosis give insight into the evolution of MetX family of enzymes - HAT, SAT and HST.结核分枝杆菌高丝氨酸乙酰转移酶中的关键氨基酸残基为 MetX 家族酶（HAT、SAT 和 HST）的进化提供了线索。
Biochimie. 2021 Oct;189:13-25. doi: 10.1016/j.biochi.2021.05.016. Epub 2021 Jun 4.
8
Purification and characterization of Thermotoga maritima homoserine transsuccinylase indicates it is a transacetylase.嗜热栖热菌高丝氨酸转琥珀酰酶的纯化与特性表明它是一种转乙酰酶。
Extremophiles. 2006 Oct;10(5):469-78. doi: 10.1007/s00792-006-0522-3. Epub 2006 May 18.
9
Nucleotide sequence of the metA gene encoding homoserine trans-succinylase in Escherichia coli.编码大肠杆菌中高丝氨酸转琥珀酰酶的metA基因的核苷酸序列。
Nucleic Acids Res. 1989 Apr 11;17(7):2856. doi: 10.1093/nar/17.7.2856.
10
A single amino acid change is responsible for evolution of acyltransferase specificity in bacterial methionine biosynthesis.单个氨基酸的变化导致了细菌甲硫氨酸生物合成中酰基转移酶特异性的进化。
J Biol Chem. 2008 Mar 21;283(12):7561-7. doi: 10.1074/jbc.M709283200. Epub 2008 Jan 22.

引用本文的文献

1
Engineering of methionine-auxotroph via parallel evolution of two enzymes from direct-sulfurylation pathway enables its recovery in minimal medium.通过直接硫酰化途径中两种酶的平行进化工程构建甲硫氨酸营养缺陷型，使其能够在基本培养基中恢复生长。
Metab Eng Commun. 2024 May 10;18:e00236. doi: 10.1016/j.mec.2024.e00236. eCollection 2024 Jun.
2
Conversion of methionine biosynthesis in Escherichia coli from trans- to direct-sulfurylation enhances extracellular methionine levels.将大肠杆菌中天冬氨酸途径的甲硫氨酸生物合成从反式转化为直接硫代化可提高细胞外甲硫氨酸水平。
Microb Cell Fact. 2023 Aug 11;22(1):151. doi: 10.1186/s12934-023-02150-x.
3
Differential Effects of Sulfur Amino Acid-Restricted and Low-Calorie Diets on Gut Microbiome Profile and Bile Acid Composition in Male C57BL6/J Mice.硫氨基酸限制和低热量饮食对雄性 C57BL6/J 小鼠肠道微生物组谱和胆汁酸组成的差异影响。
J Gerontol A Biol Sci Med Sci. 2021 Oct 13;76(11):1922-1929. doi: 10.1093/gerona/glaa270.
4
Adaptive laboratory evolution enhances methanol tolerance and conversion in engineered Corynebacterium glutamicum.适应性实验室进化增强了工程化谷氨酸棒杆菌对甲醇的耐受性和转化率。
Commun Biol. 2020 May 7;3(1):217. doi: 10.1038/s42003-020-0954-9.
5
Comparative genomics of transcriptional regulation of methionine metabolism in Proteobacteria.变形菌门中甲硫氨酸代谢转录调控的比较基因组学
PLoS One. 2014 Nov 20;9(11):e113714. doi: 10.1371/journal.pone.0113714. eCollection 2014.
6
Serinol: small molecule - big impact.丝氨醇：小分子，大影响。
AMB Express. 2011 Jun 13;1(1):12. doi: 10.1186/2191-0855-1-12.
7
The genome sequence of Geobacter metallireducens: features of metabolism, physiology and regulation common and dissimilar to Geobacter sulfurreducens.嗜金属还原地杆菌的基因组序列：与硫还原地杆菌相同和不同的代谢、生理及调控特征
BMC Microbiol. 2009 May 27;9:109. doi: 10.1186/1471-2180-9-109.
8
Catabolism of benzoate and phthalate in Rhodococcus sp. strain RHA1: redundancies and convergence.红球菌属菌株RHA1中苯甲酸酯和邻苯二甲酸酯的分解代谢：冗余性与趋同性
J Bacteriol. 2005 Jun;187(12):4050-63. doi: 10.1128/JB.187.12.4050-4063.2005.
9
Comparative genomics of the methionine metabolism in Gram-positive bacteria: a variety of regulatory systems.革兰氏阳性菌中甲硫氨酸代谢的比较基因组学：多种调控系统
Nucleic Acids Res. 2004 Jun 23;32(11):3340-53. doi: 10.1093/nar/gkh659. Print 2004.
10
Corynebacterium glutamicum utilizes both transsulfuration and direct sulfhydrylation pathways for methionine biosynthesis.谷氨酸棒杆菌利用转硫途径和直接巯基化途径进行甲硫氨酸的生物合成。
J Bacteriol. 2002 Mar;184(5):1277-86. doi: 10.1128/JB.184.5.1277-1286.2002.

本文引用的文献

1
Heat shock-dependent transcriptional activation of the metA gene of Escherichia coli.大肠杆菌metA基因的热休克依赖性转录激活。
J Bacteriol. 1995 Mar;177(5):1374-9. doi: 10.1128/jb.177.5.1374-1379.1995.
2
Whole-genome random sequencing and assembly of Haemophilus influenzae Rd.流感嗜血杆菌Rd的全基因组随机测序与组装
Science. 1995 Jul 28;269(5223):496-512. doi: 10.1126/science.7542800.
3
Feedback inhibition by methionine and S-adenosylmethionine, and desensitization of homoserine O-acetyltransferase in Brevibacterium flavum.
J Biochem. 1981 May;89(5):1493-500. doi: 10.1093/oxfordjournals.jbchem.a133342.
4
Construction and physical mapping of plasmids containing the MetA gene of Escherichia coli K-12.含有大肠杆菌K-12 MetA基因的质粒的构建及物理图谱分析
Mol Gen Genet. 1981;182(2):349-54. doi: 10.1007/BF00269682.
5
Regulatory region of the metA gene of Escherichia coli K-12.大肠杆菌K-12 metA基因的调控区域。
J Bacteriol. 1984 Dec;160(3):1158-62. doi: 10.1128/jb.160.3.1158-1162.1984.
6
The enzymic formation of O-acetylhomoserine in Bacillus subtilis and its regulation by methionine and S-adenosylmethionine.
Biochem Biophys Res Commun. 1971 Nov 5;45(3):735-41. doi: 10.1016/0006-291x(71)90478-5.
7
Amino acid biosynthesis in the spirochete Leptospira: evidence for a novel pathway of isoleucine biosynthesis.螺旋体钩端螺旋体中的氨基酸生物合成：异亮氨酸生物合成新途径的证据。
J Bacteriol. 1974 Jan;117(1):203-11. doi: 10.1128/jb.117.1.203-211.1974.
8
The MET2 gene of Saccharomyces cerevisiae: molecular cloning and nucleotide sequence.酿酒酵母的MET2基因：分子克隆与核苷酸序列
Gene. 1986;49(3):283-93. doi: 10.1016/0378-1119(86)90364-1.
9
Partial purification and some properties of homoserine O-acetyltransferase of a methionine auxotroph of Saccharomyces cerevisiae.酿酒酵母甲硫氨酸营养缺陷型高丝氨酸O-乙酰转移酶的部分纯化及某些性质
J Bacteriol. 1987 Aug;169(8):3458-63. doi: 10.1128/jb.169.8.3458-3463.1987.
10
CLUSTAL: a package for performing multiple sequence alignment on a microcomputer.CLUSTAL：一个用于在微型计算机上进行多序列比对的程序包。
Gene. 1988 Dec 15;73(1):237-44. doi: 10.1016/0378-1119(88)90330-7.