Gersten D M, Moody D, Vieira W D, Law L W, Hearing V J
Department of Pathology, Georgetown University Medical Center, Washington, DC 20007.
Biochim Biophys Acta. 1992 Feb 14;1138(2):109-14. doi: 10.1016/0925-4439(92)90049-s.
Two unique murine melanoma antigens, termed B700 and B50, have been identified and isolated from several different murine melanoma cell lines. Both antigens can be detected on the cell surface, are actively shed in culture, and are often found in close association intracellularly. In previous studies, the antigen B700, which is related to serum albumin by biochemical and immunological criteria, was shown to function as a melanoma-specific tumor rejection antigen. We have also shown that animals sensitized to irradiated JB/RH melanoma cells produce antibodies which recognize B700 and/or B50, with B700 evoking the stronger humoral response. Animals testing positive by ELISA for antibody production to B700 or B50 were used for preparation of hybridomas and four different murine monoclonal antibodies have been produced whose specificities should facilitate epitope mapping. Clones have been used to generate ascites fluid in nude mice; the antibodies specifically recognize B700 and intact murine melanoma cells, but not B50. Two of these monoclonal antibodies have been administered systemically to C57Bl/6 mice bearing 5 day pulmonary metastases of the JB/MS melanoma, and significant inhibition of metastatic growth was observed for both antibodies.
从几种不同的小鼠黑色素瘤细胞系中已鉴定并分离出两种独特的小鼠黑色素瘤抗原，分别称为B700和B50。这两种抗原均可在细胞表面检测到，在培养过程中会被主动释放，并且在细胞内常紧密相连。在先前的研究中，根据生化和免疫学标准，与血清白蛋白相关的抗原B700被证明可作为黑色素瘤特异性肿瘤排斥抗原。我们还表明，对经辐射的JB/RH黑色素瘤细胞致敏的动物会产生识别B7（此处原文有误，应为B700）和/或B50的抗体，其中B700引发更强的体液反应。通过ELISA检测对B700或B50产生抗体呈阳性的动物用于制备杂交瘤，现已产生四种不同的小鼠单克隆抗体，其特异性应有助于表位作图。已利用克隆在裸鼠体内产生腹水；这些抗体特异性识别B700和完整的小鼠黑色素瘤细胞，但不识别B50。已将其中两种单克隆抗体全身给药于携带JB/MS黑色素瘤5天肺转移灶的C57Bl/6小鼠，两种抗体均观察到对转移生长有显著抑制作用。
