Yabushita H, Masuda T, Sawaguchi K, Noguchi M, Nakanishi M
Department of Obstetrics and Gynecology, Aichi Medical University, Japan.
Gynecol Oncol. 1992 Mar;44(3):263-7. doi: 10.1016/0090-8258(92)90054-m.
Ki-67 is a monoclonal antibody directed against a nuclear antigen present only in proliferating cells. To assess the growth potential of uterine endometrial cancer, the population of cells in proliferating cycle (%PC) was examined with Ki-67, using flow cytometry. The %PC of 27.18 +/- 12.00% in 22 endometrial cancers was significantly higher than the 14.5 +/- 5.94% found in 28 normal endometrial tissues. In premenopausal endometrial tissue, the %PC in the proliferatory phase was significantly higher than the %PC found in the secretory phase. In endometrial cancers, an increase of %PC was found in cases with deep myometrial invasion, and the %PC was elevated in groups containing histologically poorly differentiated types when compared to groups of well-differentiated and moderately differentiated types. Sorted cells reactive with Ki-67 antibody were large and had a high nuclear/cytoplasmic ratio. On the bases of these results, it was concluded that a Ki-67 Ag/DNA dual-color assay would be useful to examine the growth fraction in endometrial carcinoma and that an increased growth fraction was related to deep myometrial invasion or poorly differentiated types.
Ki-67是一种针对仅存在于增殖细胞中的核抗原的单克隆抗体。为评估子宫内膜癌的生长潜能，采用流式细胞术，用Ki-67检测增殖周期中的细胞群体（%PC）。22例子宫内膜癌的%PC为27.18±12.00%，显著高于28例正常子宫内膜组织中的14.5±5.94%。在绝经前子宫内膜组织中，增殖期的%PC显著高于分泌期的%PC。在子宫内膜癌中，深肌层浸润病例的%PC增加，与高分化和中分化类型组相比，组织学低分化类型组的%PC升高。与Ki-67抗体反应的分选细胞体积大，核质比高。基于这些结果，得出结论：Ki-67抗原/DNA双色检测法对于检测子宫内膜癌的生长分数是有用的，并且生长分数增加与深肌层浸润或低分化类型有关。
