Ueda G, Sawada M, Ogawa H, Tanizawa O, Tsujimoto M
Department of Obstetrics and Gynecology, Osaka University Medical School, Japan.
Gynecol Oncol. 1993 Mar;48(3):314-6. doi: 10.1006/gyno.1993.1055.
We performed an immunohistochemical study of argyrophil cells in 10 endometrial carcinomas using the monoclonal antibody PC 10 to detect proliferating cell nuclear antigen (PCNA). Formalin-fixed, paraffin-embedded sections of 10 endometrial carcinomas containing argyrophil cells which are chromogranin positive (four containing type I cells, two type II, and four mixed-type I and type II) were stained with monoclonal antibody PC 10. In addition, one of the tumors with mixed-type cells was transplanted into nude mice, and the tumors which developed in subpassages were also analyzed for PCNA. Argyrophil cells in 10 endometrial carcinomas were found to be negative immunohistochemically for PCNA, regardless of their type, suggesting that they are not proliferating. Surprisingly, however, all subpassaged tumors contained argyrophil cells which were also negative for PCNA. From these results, it may be postulated that argyrophil cells were induced through differentiation to neuroendocrine direction of neoplastic cells.
我们使用单克隆抗体PC 10对10例子宫内膜癌中的嗜银细胞进行了免疫组织化学研究，以检测增殖细胞核抗原（PCNA）。对10例含有嗜银细胞（嗜铬粒蛋白阳性）的子宫内膜癌（其中4例含I型细胞，2例含II型细胞，4例为I型和II型混合型）的福尔马林固定、石蜡包埋切片用单克隆抗体PC 10进行染色。此外，将其中1例混合型细胞肿瘤移植到裸鼠体内，并对传代后生长的肿瘤也进行PCNA分析。结果发现，10例子宫内膜癌中的嗜银细胞无论其类型如何，免疫组织化学检测PCNA均为阴性，提示它们不增殖。然而，令人惊讶的是，所有传代肿瘤均含有嗜银细胞，其PCNA也为阴性。根据这些结果，可以推测嗜银细胞是通过肿瘤细胞向神经内分泌方向分化而诱导产生的。
