le Roy D, Expert D, Razafindratsita A, Deroussent A, Cosme J, Bohuon C, Andremont A
Laboratoire d'Ecologie Microbienne, Institut Gustave-Roussy, Villejuif, France.
Infect Immun. 1992 Mar;60(3):768-72. doi: 10.1128/iai.60.3.768-772.1992.
We isolated a monoclonal antibody directed against the ferric complex of aerobactin purified from Escherichia coli KH576. This antibody, which we designated MAb AERO1, was identified as an immunoglobulin G, subtype 2. A competitive enzyme-linked immunosorbent assay with MAb AERO1 had a limit of 10 nM for the detection of purified ferric aerobactin and allowed detection of the crude aerobactin produced by various members of the family Enterobacteriaceae isolated from cancer patients with bacteremia. The only two other structurally related siderophores recognized by MAb AERO1 were ferric arthrobactin and ferrioxamine B. These results suggest that the epitope recognized by MAb AERO1 was the lysyl moiety of ferric aerobactin. We also showed that MAb AERO1 reduced the growth of an aerobactin-producing strain of E. coli in newborn calf serum, which indicates that it might be effective in reducing the severity of infections caused by bacteria for which the production of aerobactin is an important virulence factor.
我们从大肠杆菌KH576中分离出一种针对气杆菌素铁复合物的单克隆抗体。我们将这种抗体命名为MAb AERO1，鉴定为免疫球蛋白G 2亚型。用MAb AERO1进行的竞争性酶联免疫吸附测定检测纯化的气杆菌素铁复合物的下限为10 nM，并能检测从患有菌血症的癌症患者中分离出的肠杆菌科各成员产生的粗制气杆菌素。MAb AERO1识别的仅有的其他两种结构相关的铁载体是节杆菌铁载体和去铁胺B。这些结果表明，MAb AERO1识别的表位是气杆菌素铁的赖氨酰部分。我们还表明，MAb AERO1可降低产气杆菌素的大肠杆菌菌株在新生小牛血清中的生长，这表明它可能有效地降低由气杆菌素产生是重要毒力因子的细菌引起的感染的严重程度。
