Staros J V, Wright R W, Swingle D M
Anal Biochem. 1986 Jul;156(1):220-2. doi: 10.1016/0003-2697(86)90176-4.
Water-soluble carbodiimides are frequently employed in coupling or conjugation reactions, e.g., to link a peptide immunogen to a carrier protein. However, their utility is limited by low coupling yields obtained under some conditions. We have found that addition of N-hydroxysulfosuccinimide to such reactions can greatly enhance the yields obtained.
水溶性碳二亚胺常用于偶联或缀合反应，例如，将肽免疫原与载体蛋白连接。然而，在某些条件下获得的低偶联产率限制了它们的实用性。我们发现，在此类反应中添加N-羟基磺基琥珀酰亚胺可大大提高产率。
