Soares F A
Department of Pathology, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Brazil.
J Clin Pathol. 1992 Feb;45(2):140-2. doi: 10.1136/jcp.45.2.140.
To compare the number of pulmonary megakaryocytes in patients with local malignant disease without metastases with the numbers in patients with pulmonary tumour emboli without lung metastases and with those with pulmonary metastases.
The prevalence of pulmonary megakaryocytes was studied in 40 necropsies divided into four groups of 10 cases each: normal lungs (I); localised malignancies (II); pulmonary tumour embolism without lung metastases (III); pulmonary tumour embolism and lung metastases (IV). Five fragments (one of each pulmonary lobe) of tissue lung were collected, embedded in paraffin wax, sectioned, and stained by an immunohistochemical method to detect factor VIII related antigen. The number of megakaryocytes was evaluated in 500 high power fields/case.
No differences were observed between groups I and II or between groups III and IV, but there was a 3.5-fold increase in the number of megakaryocytes in the groups with pulmonary tumour embolism or lung metastases compared with those with local neoplasms or normal lungs.
An increased number of pulmonary megakaryocytes correlated with the presence of tumour cells in the microcirculation of the lungs or parenchymal metastases, but not with local malignancies without lung disease. The permanent siting of tumour emboli may stimulate megakaryocytes to migrate to the lungs, and may increase the release of platelets into the pulmonary circulation.
比较无转移的局部恶性疾病患者、无肺转移的肺肿瘤栓子患者以及有肺转移患者的肺巨核细胞数量。
在40例尸检中研究肺巨核细胞的患病率，将其分为四组，每组10例：正常肺（I组）；局部恶性肿瘤（II组）；无肺转移的肺肿瘤栓子（III组）；肺肿瘤栓子和肺转移（IV组）。收集五个肺组织碎片（每个肺叶一个），石蜡包埋，切片，并采用免疫组织化学方法染色以检测因子VIII相关抗原。每例在500个高倍视野中评估巨核细胞数量。
I组和II组之间以及III组和IV组之间未观察到差异，但与局部肿瘤或正常肺患者相比，有肺肿瘤栓子或肺转移的组中巨核细胞数量增加了3.5倍。
肺巨核细胞数量增加与肺微循环中肿瘤细胞的存在或实质转移相关，但与无肺部疾病的局部恶性肿瘤无关。肿瘤栓子的长期停留可能刺激巨核细胞迁移至肺，并可能增加血小板向肺循环的释放。
