Gerena R L, Killian G J
Dairy Breeding Research Center, Pennsylvania State University, University Park 16802.
J Exp Zool. 1990 Oct;256(1):113-20. doi: 10.1002/jez.1402560114.
This study was undertaken to examine the in vivo protein composition of the bovine oviduct during the estrous cycle. Oviduct fluid was collected daily from four oviduct-cannulated dairy cows for a total of four complete estrous cycles. Fluid secretion followed a definite cyclic pattern, with maximum secretion occurring at estrus in all cycles. Protein concentration fluctuated during the cycle and varied among animals. In general, protein concentration was lower at the time of estrus. Total protein in oviduct fluid, however, was higher around estrus, indicating increased transudation or secretion by the oviduct. One-dimensional SDS-PAGE separation revealed the protein pattern of oviduct fluid to be generally similar to that of blood serum and follicular fluid. Two proteins appeared to be oviduct-specific. The first, a protein of approximately 47 kDa, was evident in oviduct fluid throughout the estrous cycle. The second protein, evident as a broad diffuse staining band above albumin, appeared for only 3-4 days at or near ovulation. This protein had a molecular weight of 80-95 kDa and stained positive for carbohydrate with periodic acid-Schiff reagent. These studies indicate that the in vivo protein composition of oviduct fluid varies with the estrous cycle, and that around estrus, an oviduct-specific glycoprotein is present.
本研究旨在检测发情周期中牛输卵管的体内蛋白质组成。每天从四只输卵管插管的奶牛收集输卵管液，共进行四个完整的发情周期。液体分泌遵循一定的周期性模式，在所有周期中，发情期分泌量最大。蛋白质浓度在周期中波动，且不同动物之间存在差异。一般来说，发情期时蛋白质浓度较低。然而，输卵管液中的总蛋白在发情期前后较高，表明输卵管的渗出或分泌增加。一维SDS-PAGE分离显示，输卵管液的蛋白质模式与血清和卵泡液的蛋白质模式总体相似。有两种蛋白质似乎是输卵管特有的。第一种是一种约47 kDa的蛋白质，在整个发情周期的输卵管液中都很明显。第二种蛋白质表现为白蛋白上方一条宽的弥散染色带，仅在排卵时或排卵前后出现3 - 4天。这种蛋白质的分子量为80 - 95 kDa，用高碘酸-希夫试剂染色显示碳水化合物呈阳性。这些研究表明，输卵管液的体内蛋白质组成随发情周期而变化，并且在发情期前后，存在一种输卵管特异性糖蛋白。
