Tachibana K, Chen G J, Huang D S, Scuderi P, Watson R R
Department of Fishery Food Sciences, Faculty of Fisheries, Nagasaki University, Japan.
J Leukoc Biol. 1992 Mar;51(3):251-5. doi: 10.1002/jlb.51.3.251.
Alveolar macrophages (Am phi s), resident peritoneal macrophages (RPm phi s), and thioglycolate-elicited peritoneal macrophages (TGPm phi s) were isolated from C57BL/6 mice and incubated with lipopolysaccharide (LPS), stimulated cell supernatant, or recombinant interferon gamma (IFN-gamma) for 24 h. Tumor necrosis factor (TNF) in cell-free supernatants was measured by enzyme-linked immunosorbent assay. Amo phi s incubated with 10(3) ng/ml LPS produced 50 times more TNF than RPm phi s and 5 times more than TGPm phi s, and LPS alone induced maximum TNF production by Am phi s. Stimulated cell supernatant or recombinant IFN-gamma alone did not induce TNF production. A combination of LPS with stimulated cell supernatant or IFN-gamma had only a limited synergistic effect on TNF production by Am phi s. However, both LPS and stimulated cell supernatant or recombinant IFN-gamma induced maximum TNF production by RPm phi s and TGPm phi s. TGPm phi s showed greater sensitivity to LPS and stimulated cell supernatant or IFN-gamma with regard to TNF production than the other macrophage populations investigated.
从C57BL/6小鼠中分离出肺泡巨噬细胞（Am phi s）、常驻腹膜巨噬细胞（RPm phi s）和巯基乙酸盐诱导的腹膜巨噬细胞（TGPm phi s），并与脂多糖（LPS）、刺激细胞上清液或重组干扰素γ（IFN-γ）一起孵育24小时。通过酶联免疫吸附测定法测量无细胞上清液中的肿瘤坏死因子（TNF）。与10(3) ng/ml LPS孵育的Am phi s产生的TNF比RPm phi s多50倍，比TGPm phi s多5倍，并且单独的LPS诱导Am phi s产生最大量的TNF。单独的刺激细胞上清液或重组IFN-γ不会诱导TNF产生。LPS与刺激细胞上清液或IFN-γ的组合对Am phi s产生TNF的协同作用有限。然而，LPS和刺激细胞上清液或重组IFN-γ均诱导RPm phi s和TGPm phi s产生最大量的TNF。就TNF产生而言，TGPm phi s对LPS、刺激细胞上清液或IFN-γ的敏感性高于所研究的其他巨噬细胞群体。
