Hokama Y, Hong T W, Isobe M, Ichikawa Y, Yasumoto T
Department of Pathology, JABSOM, University of Hawaii, Honolulu 96822.
J Clin Lab Anal. 1992;6(1):54-8. doi: 10.1002/jcla.1860060111.
This study presents data from the cross-reactivity analysis of purified ciguatoxin (CTX), okadaic acid (OA), and the East sphere or Fragment B-C of OA with their homologous antibodies, monoclonal antibodies to ciguatoxin (MAb-CTX) and okadaic acid (MAb-OA). The test system used was the stick enzyme immunoassay. MAb-CTX gave peak titers of 1.5ng, 10ng and 50ng respectively for CTX, East sphere and OA. Competitive inhibition analysis showed that 4ng purified CTX blocked completely MAb-CTX reaction with crude CTX, OA and East sphere of OA blocked at similar concentrations (approximately 50ng). The activity with MAb-OA in the homologous system with OA and East sphere was insignificant. This may be attributable to the improper concentrations used. The cross-reactivity between MAb-CTX with OA and its Fragment B-C may cause difficulty in the test system in its application to assess toxic fish due to ciguatoxin.
本研究展示了纯化的雪卡毒素（CTX）、冈田酸（OA）以及OA的东部区域或B - C片段与它们的同源抗体，即雪卡毒素单克隆抗体（MAb - CTX）和冈田酸单克隆抗体（MAb - OA）的交叉反应分析数据。所使用的检测系统为酶联免疫吸附试验。MAb - CTX对CTX、OA东部区域和OA的峰值效价分别为1.5纳克、10纳克和50纳克。竞争性抑制分析表明，4纳克纯化的CTX完全阻断了MAb - CTX与粗制CTX的反应，OA和OA东部区域在相似浓度（约50纳克）时被阻断。在与OA和东部区域的同源系统中，MAb - OA的活性不显著。这可能归因于所使用的浓度不合适。MAb - CTX与OA及其B - C片段之间的交叉反应可能会给该检测系统在应用于评估因雪卡毒素导致的有毒鱼类时带来困难。
