Campora Cara E, Hokama Y, Ebesu Joanne S M
Department of Cell and Molecular Biology, University of Hawaii-Manoa, Honolulu, Hawaii, USA.
J Clin Lab Anal. 2006;20(3):121-5. doi: 10.1002/jcla.20113.
The monoclonal antibody to ciguatoxin (CTX) produced from a hybridoma cell line was assayed for the detection of four congeners of CTX: Pacific ciguatoxin-1 (P-CTX-1), Pacific ciguatoxin-2 (P-CTX-2), Pacific ciguatoxin-3 (P-CTX-3), and Caribbean ciguatoxin-1 (C-CTX-1) and related marine toxins, including domoic acid, palytoxin, and okadaic acid, using a modified enzyme-linked immunosorbent assay (ELISA). Lower detection limits were assessed and linearity was statistically established (P<0.05) for P-CTX-1, P-CTX-2, and P-CTX-3 and C-CTX-1 at concentrations ranging from 0 to 5.00 ng, while the other marine toxins showed statistically insignificant cross-reactivities at similar concentrations. Thus, the monoclonal antibody to CTX is able to specifically detect various CTX congeners at levels comparable to those naturally occurring in ciguatoxic fish.
使用改良的酶联免疫吸附测定法（ELISA），对源自杂交瘤细胞系的西加毒素（CTX）单克隆抗体进行检测，以检测CTX的四种同系物：太平洋西加毒素-1（P-CTX-1）、太平洋西加毒素-2（P-CTX-2）、太平洋西加毒素-3（P-CTX-3）和加勒比西加毒素-1（C-CTX-1）以及相关海洋毒素，包括软骨藻酸、岩沙海葵毒素和冈田酸。评估了最低检测限，并在浓度范围为0至5.00 ng时，对P-CTX-1、P-CTX-2、P-CTX-3和C-CTX-1进行了线性统计分析（P<0.05），而其他海洋毒素在相似浓度下显示出统计学上无显著意义的交叉反应性。因此，CTX单克隆抗体能够特异性检测各种CTX同系物，其水平与西加毒鱼中天然存在的水平相当。
