DALY M M, ALLFREY V G, MIRSKY A E
J Gen Physiol. 1950 May 20;33(5):497-510. doi: 10.1085/jgp.33.5.497.
The distribution of purines and pyrimidines in desoxypentose nucleic acids prepared from a variety of animal and plant sources has been studied. 1. The nucleic acids were prepared from calf thymus, calf kidney, sheep spleen, horse spleen, chicken erythrocyte, turtle erythrocyte, trout sperm, shad testes, sea urchin sperm, wheat germ, and Pneumococcus Type III. 2. Separate hydrolyses were carried out for the determination of purines and pyrimidines. These procedures permitted nearly quantitative recovery of nucleic acid phosphorus in many of the preparations examined. 3. In the case of those preparations where a quantitative recovery was obtained it can be concluded that no bases other than adenine, guanine, thymine, and cytosine were present in appreciable amounts. 4. The distribution of purines and pyrimidines in all the nucleic acids studied renders the tetranucleotide hypothesis untenable. 5. The results of the analyses have indicated no great differences in the composition of these nucleic acids with respect to purines and pyrimidines.
对从多种动植物来源制备的脱氧戊糖核酸中嘌呤和嘧啶的分布进行了研究。1. 核酸取自小牛胸腺、小牛肾脏、绵羊脾脏、马脾脏、鸡红细胞、海龟红细胞、鳟鱼精子、鲱鱼睾丸、海胆精子、小麦胚芽和III型肺炎球菌。2. 为测定嘌呤和嘧啶进行了单独水解。这些步骤使许多检测的制剂中核酸磷几乎能定量回收。3. 在那些获得定量回收的制剂中，可以得出结论，除腺嘌呤、鸟嘌呤、胸腺嘧啶和胞嘧啶外，不存在大量其他碱基。4. 所研究的所有核酸中嘌呤和嘧啶的分布使四核苷酸假说难以成立。5. 分析结果表明，这些核酸在嘌呤和嘧啶组成方面没有很大差异。
