一个在布氏锥虫和 fasciculata 克氏锥虫之间保守的磷酸甘油酸激酶相关基因。 （注：这里“Crithidia fasciculata”常见的中译名是“纤细克氏锥虫” ，但原译文表述不太准确，更准确的完整译文应该是：一个在布氏锥虫和纤细克氏锥虫之间保守的磷酸甘油酸激酶相关基因。 ）
A phosphoglycerate kinase-related gene conserved between Trypanosoma brucei and Crithidia fasciculata.
作者信息
Swinkels B W, Loiseau A, Opperdoes F R, Borst P
机构信息
Division of Molecular Biology, The Netherlands Cancer Institute, Amsterdam.
出版信息
Mol Biochem Parasitol. 1992 Jan;50(1):69-78. doi: 10.1016/0166-6851(92)90245-f.
Trypanosoma brucei and Crithidia fasciculata both contain three different phosphoglycerate kinase (PGK) genes, A, B and C, in a tandem array. The genes B and C encode the major PGKs: the cytosolic and glycosomal PGKs, respectively. The PGK-A genes of both Trypanosomatid species encode open reading frames related to PGK, which have most active site residues conserved, but contain an insert of 80 amino acids at approximately position 80 of the 420 amino acids average PGK sequence. The deduced amino acid sequence of these inserts is conserved between T. brucei and C. fasciculata (48% positional identity), indicating its functional importance. Although we have not been able to demonstrate PGK activity in the PGK-A gene product, we consider it likely that this gene codes for a minor PGK with special function.
布氏锥虫和 fasciculata 短膜虫都在串联阵列中包含三个不同的磷酸甘油酸激酶（PGK）基因，A、B 和 C。基因 B 和 C 分别编码主要的 PGK：胞质 PGK 和糖体 PGK。这两种锥虫物种的 PGK-A 基因编码与 PGK 相关的开放阅读框，其大多数活性位点残基保守，但在平均 420 个氨基酸的 PGK 序列的大约第 80 位含有 80 个氨基酸的插入序列。这些插入序列的推导氨基酸序列在布氏锥虫和 fasciculata 短膜虫之间是保守的（48%的位置同一性），表明其功能重要性。尽管我们未能在 PGK-A 基因产物中证明 PGK 活性，但我们认为该基因可能编码具有特殊功能的次要 PGK。
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