Use of oligonucleotides and nick translation for site-directed mutagenesis in plasmids.
作者信息
Drutsa V L, Kaberdin V R
机构信息
A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Russia.
出版信息
Nucleic Acids Res. 1992 Feb 25;20(4):922. doi: 10.1093/nar/20.4.922.
Abstract
摘要
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Oligonucleotide-directed mutagenesis using plasmid DNA templates and two primers.使用质粒DNA模板和两种引物的寡核苷酸定向诱变。
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Convenient modification of the method for oligonucleotide-directed in vitro mutagenesis of cloned DNA.克隆DNA的寡核苷酸定向体外诱变方法的简便改进。
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Generation of single-stranded DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DQA locus.通过聚合酶链反应生成单链DNA及其在HLA - DQA基因座直接测序中的应用。
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A rapid and versatile site-directed method of mutagenesis for double-stranded plasmid DNA.
Gene. 1988 Sep 30;69(2):325-30. doi: 10.1016/0378-1119(88)90442-8.
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DNA. 1987 Jun;6(3):273-9. doi: 10.1089/dna.1987.6.273.
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In vivo promoter activity of the synthetic Pribnow box.
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