Efimov V A, Mirskikh O V, Chakhmakhcheva O G
FEBS Lett. 1985 Feb 25;181(2):407-11. doi: 10.1016/0014-5793(85)80302-1.
A new modification of the oligonucleotide-mediated mutagenesis technique has been developed. The proposed methodology has been used to produce specific base changes in the double-stranded plasmid DNA. For this purpose, special cloning vectors have been constructed using the synthetic oligodeoxyribonucleotides. The developed method allows the production of mutant DNA from those of the wild-type with a yield of 10-20%.
已经开发出一种寡核苷酸介导的诱变技术的新改良方法。所提出的方法已用于在双链质粒DNA中产生特定的碱基变化。为此，使用合成的寡脱氧核糖核苷酸构建了特殊的克隆载体。所开发的方法能够以10%-20%的产率从野生型DNA产生突变体DNA。
