Koroleva O N, Drutsa V L
A.N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, USSR.
FEBS Lett. 1991 Jan 28;278(2):207-10. doi: 10.1016/0014-5793(91)80118-m.
The synthetic polydeoxyribonucleotides TGCA(TTATAATGCA)1-4, containing the consensus Pribnow box, were inserted upstream of the promoterless gal operon in a specially constructed plasmid. Using the MacConkey agar indicator technique it has been shown that these inserts are able to induce an efficient in vivo expression of the gal operon.