Nair B C, DeVico A L, Nakamura S, Copeland T D, Chen Y, Patel A, O'Neil T, Oroszlan S, Gallo R C, Sarngadharan M G
Department of Cell Biology, Advanced BioScience Laboratories, Inc., Kensington, MD.
Science. 1992 Mar 13;255(5050):1430-2. doi: 10.1126/science.1542792.
Conditioned medium from human T cell leukemia virus type 2 (HTLV-II)-infected T cells supports the growth and long-term culture of cells derived from acquired immunodeficiency syndrome (AIDS)-associated Kaposi's sarcoma lesions (AIDS-KS cells). A protein of 30 kilodaltons was purified from conditioned medium that supports the growth of AIDS-KS cells. The amino-terminal sequence of this protein was identical to the amino-terminal sequence of Oncostatin M, a glycoprotein that inhibits the growth of a variety of cancer cells. Oncostatin M from conditioned medium stimulated a twofold increase in the growth of AIDS-KS cells at a concentration of less than 1 nanogram of the protein per milliliter of medium.
来自人类2型T细胞白血病病毒（HTLV-II）感染的T细胞的条件培养基支持源自获得性免疫缺陷综合征（AIDS）相关卡波西肉瘤病变的细胞（AIDS-KS细胞）的生长和长期培养。从支持AIDS-KS细胞生长的条件培养基中纯化出一种30千道尔顿的蛋白质。该蛋白质的氨基末端序列与制瘤素M的氨基末端序列相同，制瘤素M是一种抑制多种癌细胞生长的糖蛋白。条件培养基中的制瘤素M在浓度低于每毫升培养基1纳克蛋白质时，可刺激AIDS-KS细胞的生长增加两倍。
