Weniger J P, Cate R L, Zeis A
Laboratorie de Zoologie et d'Embryologie Expérimentale, Université Louis Pasteur, Strasbourg, France.
J Mol Endocrinol. 1992 Feb;8(1):73-7. doi: 10.1677/jme.0.0080073.
To determine whether mammalian Müllerian-inhibiting substance (MIS) is active in birds, Müllerian ducts from 7- to 8-day-old male or female chick embryos were cultured in the presence of human recombinant MIS at concentrations between 2.5 and 12.5 micrograms/ml. None of 20 ducts regressed at any concentration. In contrast, at concentrations of 2.5-5 micrograms/ml, all 12 Müllerian ducts from 13-day-old male mouse embryos and 13 out of 14 female ducts were inhibited to varying degrees. It is concluded that avian Müllerian ducts are unresponsive to mammalian MIS. There may be a difference in structure between the MIS of birds and mammals, or the signal-transduction system may be different.
为了确定哺乳动物苗勒氏管抑制物质（MIS）在鸟类中是否具有活性，将7至8日龄雄性或雌性鸡胚胎的苗勒氏管在浓度为2.5至12.5微克/毫升的重组人MIS存在下进行培养。在任何浓度下，20条苗勒氏管均未发生退化。相比之下，在浓度为2.5至5微克/毫升时，来自13日龄雄性小鼠胚胎的12条苗勒氏管以及14条雌性苗勒氏管中的13条均受到不同程度的抑制。结论是禽类苗勒氏管对哺乳动物MIS无反应。鸟类和哺乳动物的MIS在结构上可能存在差异，或者信号转导系统可能不同。
