Ikawa H, Hutson J M, Budzik G P, MacLaughlin D T, Donahoe P K
J Pediatr Surg. 1982 Oct;17(5):453-8. doi: 10.1016/s0022-3468(82)80088-2.
The biologic activity of the glycoprotein, Mullerian Inhibiting Substance (MIS), was enhanced by added testosterone, medroxyprogesterone acetate, and progesterone, although these steroids did not cause Mullerian duct regression when added alone. Statistically significant augmentation by testosterone and medroxyprogesterone was observed at concentrations of 10(-7) and 10(-8) M. Progesterone enhanced MIS activity at a concentration of 10(-6) M. By contrast, dihydrotestosterone, estradiol, and corticosterone at concentrations of 10(-9) to 10(-5) M produced no significant effect on MIS activity. Since MIS does not bind directly to the steroids studied, we suspect that the enhancement effect occurs at the receptor level, probably at the mesenchyme as observed in other developmental systems.
缪勒管抑制物质（MIS）这种糖蛋白的生物活性可被添加的睾酮、醋酸甲羟孕酮和孕酮增强，尽管这些类固醇单独添加时并不会导致缪勒管退化。在浓度为10⁻⁷和10⁻⁸ M时，观察到睾酮和醋酸甲羟孕酮有统计学意义的增强作用。孕酮在浓度为10⁻⁶ M时增强了MIS活性。相比之下，浓度为10⁻⁹至10⁻⁵ M的双氢睾酮、雌二醇和皮质酮对MIS活性没有显著影响。由于MIS并不直接与所研究的类固醇结合，我们推测增强作用发生在受体水平，可能像在其他发育系统中观察到的那样，发生在间充质。
