链球菌溶血素S的纯化及特性
The purification and properties of streptolysin S.
作者信息
CINADER B, PILLEMER L
出版信息
J Exp Med. 1950 Sep;92(3):219-37. doi: 10.1084/jem.92.3.219.
Abstract
Considerable purification of streptolysin S has been achieved by fractionation of crude bacterial filtrates with methanol, under controlled conditions of pH, ionic strength, and temperature. The final material (P III) consisted of two electrophoretic components. The purified material was employed for kinetic studies, the results of which were confirmed with hemolysin isolated electrophoretically from P III. The heat of activation (temperature characteristic) of streptolysin S was found to be 17,900 calories per mole. Time dilution curves of the hemolysin were found to be sigmoid. The influence of certain ions and lipids on the course of hemolysis was investigated. The significance of the prolonged lag period and of the deviation in the behavior of streptolysin S from Ponder's equation has been discussed.
摘要
通过在pH、离子强度和温度可控的条件下，用甲醇对粗制细菌滤液进行分级分离，已实现了链球菌溶血素S的大量纯化。最终产物（P III）由两个电泳成分组成。纯化后的物质用于动力学研究，其结果通过从P III中电泳分离出的溶血素得到了证实。发现链球菌溶血素S的活化热（温度特性）为每摩尔17,900卡路里。溶血素的时间稀释曲线呈S形。研究了某些离子和脂质对溶血过程的影响。讨论了链球菌溶血素S延长的延迟期以及其行为偏离庞德方程的意义。
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1
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J Exp Med. 1944 Oct 1;80(4):333-9. doi: 10.1084/jem.80.4.333.
2
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J Exp Med. 1927 Oct 31;46(5):755-75. doi: 10.1084/jem.46.5.755.
3
The effect of nucleic acids and of carbohydrates on the formation of streptolysin.核酸和碳水化合物对链球菌溶血素形成的影响。
J Exp Med. 1948 Aug;88(2):149-68. doi: 10.1084/jem.88.2.149.
4
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Biochem J. 1941 Sep;35(8-9):884-902. doi: 10.1042/bj0350884.
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6
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