Sourcing and batch differences are often cited as intrinsic drawbacks for all natural polymers. Chitosan makes no exception. Chitosan is a biocompatible and biodegradable biopolymer with high potential for several biomedical applications, especially for releasing drugs and bactericidal and virucidal agents. Despite the potential of chitosan as a matrix for producing antibacterial films, the variability in its composition, stemming from its natural sources, can hinder the translation from bench to industry. To overcome this concern, we conducted a study to access the interchangeability of chitosan for the development of antibacterial drug release systems, in particular one system crosslinked with tannic acid and iron sulfate. Chitosans from different suppliers were characterized and used to synthetize films containing gentamicin, according to a previously reported protocol. The impact of molecular weight (M), deacetylation degree and purity on film properties and antibiotic release kinetics was assessed and results were compared. The films exhibited different initial bursts followed by similar sustained release profiles. All films exhibited antibacterial activity against both and for at least 42 days. Moreover, films were cyto- and hemocompatible. Therefore, despite some differences in physicochemical properties, the interchangeability among the studied chitosan suppliers to produce antibacterial films is feasible, and the final product properties and performances are not significantly altered.