剑尾鱼的微卫星遗传连锁图谱。
A microsatellite genetic linkage map for Xiphophorus.
作者信息
Walter R B, Rains J D, Russell J E, Guerra T M, Daniels C, Johnston Dennis A, Kumar Jay, Wheeler A, Kelnar K, Khanolkar V A, Williams E L, Hornecker J L, Hollek L, Mamerow M M, Pedroza A, Kazianis S
机构信息
Molecular Biosciences Research Group, Department of Chemistry and Biochemistry, Texas State University, San Marcos 78666, USA.
出版信息
Genetics. 2004 Sep;168(1):363-72. doi: 10.1534/genetics.103.019349.
Interspecies hybrids between distinct species of the genus Xiphophorus are often used in varied research investigations to identify genomic regions associated with the inheritance of complex traits. There are 24 described Xiphophorus species and a greater number of pedigreed strains; thus, the number of potential interspecies hybrid cross combinations is quite large. Previously, select Xiphophorus experimental crosses have been shown to exhibit differing characteristics between parental species and among the hybrid fishes derived from crossing them, such as widely differing susceptibilities to chemical or physical agents. For instance, genomic regions harboring tumor suppressor and oncogenes have been identified via linkage association of these loci with a small set of established genetic markers. The power of this experimental strategy is related to the number of genetic markers available in the Xiphophorus interspecies cross of interest. Thus, we have undertaken the task of expanding the suite of easily scored markers by characterization of Xiphophorus microsatellite sequences. Using a cross between Xiphophorus maculatus and X. andersi, we report a linkage map predominantly composed of microsatellite markers. All 24 acrocentric chromosome sets of Xiphophorus are represented in the assembled linkage map with an average intergenomic distance of 7.5 cM. Since both male and female F1 hybrids were used to produce backcross progeny, these recombination rates were compared between "male" and "female" maps. Although several genomic regions exhibit differences in map length, male- and female-derived maps are similar. Thus Xiphophorus, in contrast to zebrafish, Danio rerio, and several other vertebrate species, does not show sex-specific differences in recombination. The microsatellite markers we report can be easily adapted to any Xiphophorus interspecies and some intraspecies crosses, and thus provide a means to directly compare results derived from independent experiments.
剑尾鱼属不同物种之间的种间杂种常被用于各种研究调查，以确定与复杂性状遗传相关的基因组区域。已描述的剑尾鱼物种有24种，还有更多的纯种品系；因此，潜在的种间杂交组合数量相当大。此前，已表明某些剑尾鱼实验杂交在亲本物种之间以及杂交产生的杂种鱼之间表现出不同的特征，例如对化学或物理因子的易感性差异很大。例如，通过将这些基因座与一小部分已确立的遗传标记进行连锁关联，已鉴定出含有肿瘤抑制基因和致癌基因的基因组区域。这种实验策略的效力与所关注的剑尾鱼种间杂交中可用的遗传标记数量有关。因此，我们承担了通过对剑尾鱼微卫星序列进行特征分析来扩展易于评分的标记组的任务。利用剑尾鱼（Xiphophorus maculatus）和安氏剑尾鱼（X. andersi）之间的杂交，我们报告了一个主要由微卫星标记组成的连锁图谱。在组装好的连锁图谱中代表了剑尾鱼所有24个近端着丝粒染色体组，平均基因组间距离为7.5厘摩。由于雄性和雌性F1杂种都用于产生回交后代，因此对“雄性”和“雌性”图谱之间的这些重组率进行了比较。尽管几个基因组区域在图谱长度上存在差异，但雄性和雌性来源的图谱相似。因此，与斑马鱼、丹氏斑马鱼（Danio rerio）和其他几种脊椎动物物种不同，剑尾鱼在重组方面没有表现出性别特异性差异。我们报告的微卫星标记可以很容易地应用于任何剑尾鱼种间杂交以及一些种内杂交，从而提供了一种直接比较来自独立实验结果的方法。
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