Dawson G Brent, Matyska Maria T, Pesek Joseph J, Seipert Richard R
Department of Chemistry, University of North Carolina, Greensboro, Greensboro, NC 27403, USA.
J Chromatogr A. 2004 Aug 27;1047(2):299-303.
A liquid crystal stationary phase for open tubular capillary electrochromatograpy (OTCEC) is fabricated by etching a fused silica tube and then bonding 4,4'-cyanopentoxy biphenyl by a silanization/hydrosilation process. The versatility of this electrophoretic capillary is demonstrated by separations of proteins, peptides, basic pharmaceuticals and the metabolites of tryptophan. Chromatographic interactions are verified by resolution of two neutral peptides. Variable temperature studies are used to understand the liquid crystal properties of the bonded moiety. EOF measurements as a function of pH and temperature further characterize this unique separation media.
一种用于开管毛细管电色谱（OTCEC）的液晶固定相，是通过蚀刻熔融石英管，然后通过硅烷化/硅氢化过程键合4,4'-氰基戊氧基联苯来制备的。这种电泳毛细管的多功能性通过蛋白质、肽、碱性药物和色氨酸代谢物的分离得到了证明。通过两种中性肽的分离验证了色谱相互作用。采用可变温度研究来了解键合部分的液晶性质。作为pH和温度函数的电渗流（EOF）测量进一步表征了这种独特的分离介质。
