Pesek Joseph J, Matyska Maria T, Velpula Sunandini
Department of Chemistry, San Jose State University, San Jose, CA 95192, USA.
J Sep Sci. 2005 May;28(8):746-50. doi: 10.1002/jssc.200400100.
Capillaries for use in electrophoretic analyses are etched with ammonium bifluoride and in some cases a second inorganic salt is included in the process. The effects of the presence of these inorganic components in the surface matrix on the electromigration of heterocyclic aromatic amines and enkephalins are evaluated. Resolution, efficiency, and peak shape are used to compare the various columns. In one instance, the etched surface is then modified by the addition of an octadecyl moiety using a silanization/hydrosilation procedure. The surface properties of the various etched capillaries are also compared by electroosmotic flow measurements.
用于电泳分析的毛细管要用氟化氢铵进行蚀刻，在某些情况下，该过程中还会加入第二种无机盐。评估了表面基质中这些无机成分的存在对杂环芳香胺和脑啡肽电迁移的影响。使用分离度、柱效和峰形来比较各种色谱柱。在一个实例中，然后采用硅烷化/硅氢化程序通过添加十八烷基部分对蚀刻后的表面进行改性。还通过电渗流测量比较了各种蚀刻毛细管的表面性质。
