Zhang P, Fegan N, Fraser I, Duffy P, Bowles R E, Gordon A, Ketterer P J, Shinwari W, Blackall P J
Institute for Animal Husbandry and Veterinary Science, Beijing Academy of Agricultural and Forestry Science, Beijing, PR China.
J Vet Diagn Invest. 2004 Sep;16(5):458-60. doi: 10.1177/104063870401600517.
Two outbreaks of fowl cholera on a multiage free-range egg farm were investigated. The outbreaks occurred in 1994 and 2002. A total of 22 strains of Pasteurella multocida were available for study, 11 from the 1994 outbreak and 11 from the 2002 outbreak. Lesions typical of acute fowl cholera were seen in the 1994 outbreak, whereas both acute and chronic fowl cholera occurred in the 2002 outbreak. The isolates were examined in an extended phenotypic typing methodology, by a P. multocida-specific polymerase chain reaction (PCR), by the Heddleston somatic serotyping scheme, and by restriction endonuclease analysis (REA) typing using the enzyme HpaII. All 22 strains had the same phenotypic properties, all were confirmed as P. multocida by PCR, all were Heddleston serovar 4, and all had the same REA pattern. The results indicate that these 2 outbreaks were caused by the same clone of P. multocida--despite the 8-year time period between the outbreaks.
对一个多龄散养蛋鸡场发生的两起禽霍乱疫情进行了调查。疫情分别发生在1994年和2002年。共有22株多杀性巴氏杆菌可供研究，其中11株来自1994年的疫情，11株来自2002年的疫情。在1994年的疫情中可见典型的急性禽霍乱病变，而在2002年的疫情中急性和慢性禽霍乱均有发生。采用扩展的表型分型方法、多杀性巴氏杆菌特异性聚合酶链反应（PCR）、赫德尔斯顿菌体血清分型方案以及使用HpaII酶的限制性内切酶分析（REA）分型对分离株进行检测。所有22株菌株具有相同的表型特性，通过PCR均确认为多杀性巴氏杆菌，均为赫德尔斯顿血清型4，且具有相同的REA图谱。结果表明，尽管两次疫情间隔8年，但这两起疫情均由同一克隆的多杀性巴氏杆菌引起。
