Huang Pei-jun, Xu Pei-yu, Tao Tao, Li Lei-bo, He Gui-qiong, Xing Yu, Liao Zhi-gang
Department of Forensic Pathology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2004 Sep;35(5):644-6.
This study sought to reveal the relationship between Snap-25 and tetramine poisoning in order to approach the mechanism of tetramine poisoning.
The levels of Snap-25 mRNA in the brain tissues of poisoned SD rats were detected by RT-PCR method.
After 1 hour of intoxication, the expression level of Snap-25 mRNA did not change in the rat brain. It increased at day 1 and reached its peak at day 3. The expression level of Snap-25 mRNA begain to descend at day 5, but it was still higher than that in the rat brain tissue of control group.
In this study the expression of Snap-25 of rat poisoned by tetramine was not markedly increased in the early stage of poisoning, indicating that the symptoms of rat poisoned by tetramine are not induced by the changes of the Snap-25 level. The mechanism of high expression in Snap-25 mRNA probably involves the negative feedback of the body, which may increase the releasing of neurohumor and hence mitigate the symptoms of poisoning.
本研究旨在揭示突触小体相关蛋白25（Snap-25）与毒鼠强中毒之间的关系，以探讨毒鼠强中毒的机制。
采用逆转录聚合酶链反应（RT-PCR）法检测中毒SD大鼠脑组织中Snap-25 mRNA的水平。
中毒1小时后，大鼠脑组织中Snap-25 mRNA的表达水平未发生变化。第1天升高，第3天达到峰值。第5天Snap-25 mRNA的表达水平开始下降，但仍高于对照组大鼠脑组织中的水平。
本研究中毒鼠强中毒大鼠在中毒早期Snap-25的表达未明显增加，表明毒鼠强中毒大鼠的症状不是由Snap-25水平的变化所诱发。Snap-25 mRNA高表达的机制可能涉及机体的负反馈，这可能增加神经递质的释放，从而减轻中毒症状。
