Wang Ye, Shen Li-Hua, Chen Wei-Jie, Liu Min, Li Fan, Liao Zhi-Gang
West China Preclinical and Forensic Medicine School, Sichuan University, Chengdu 610041, China.
Fa Yi Xue Za Zhi. 2006 Aug 15;22(4):241-4.
To find whether Bcl-2 and Caspase-3 take part in the pathophysiological mechanism of tetramine toxification.
Sixty Sprague-Dawley rats were divided into normal control group, the sham poisoned group, high dose poisoned group, low dose poisoned group. High dose poisoned group were administered 1.0 mg/kg weight body tetramine by mouth, however low dose poisoned group was administered tetramine 0.1 mg/kg weight body by mouth. The rats of the sham poisoned group were administered water, and rats of normal control group were given nothing. Bcl-2 and Caspase-3 were detected by immunohistochemistry staining and the results were assessed by image analysis system.
The expressions of Bcl-2 and Caspase-3 in all organs were similar, ie, Bcl-2 and Caspase-3 expressed obviously in all organs of high dose poisoned group; in all organs of low dose poisoned group, they were hardly detected at 30 min after administration, however, at 3 h after administration, they could be detected obviously; Bcl-2 got to peak at 6 h-3 d after administration and Caspase-3 got to peak at 24 h-3 d after administration.
Bcl-2 and Caspase-3 take part in the pathophysiological procedure of tetramine poisoned rats.
探讨Bcl-2和Caspase-3是否参与毒鼠强中毒的病理生理机制。
将60只Sprague-Dawley大鼠分为正常对照组、假中毒组、高剂量中毒组、低剂量中毒组。高剂量中毒组经口给予毒鼠强1.0mg/kg体重，低剂量中毒组经口给予毒鼠强0.1mg/kg体重。假中毒组大鼠给予水，正常对照组大鼠不给予任何处理。采用免疫组织化学染色检测Bcl-2和Caspase-3，并通过图像分析系统评估结果。
Bcl-2和Caspase-3在各器官中的表达情况相似，即高剂量中毒组各器官中Bcl-2和Caspase-3均明显表达；低剂量中毒组给药30分钟时各器官中几乎检测不到，给药3小时时可明显检测到；给药后6小时至3天Bcl-2达峰值，给药后24小时至3天Caspase-3达峰值。
Bcl-2和Caspase-3参与了毒鼠强中毒大鼠的病理生理过程。
