Allen Margaret L, Christensen Bruce M
Entomology Department, University of California, Riverside, CA 92521, USA.
Parasitol Int. 2004 Dec;53(4):307-14. doi: 10.1016/j.parint.2004.04.002.
A strategy to engineer a strain of Culex mosquitoes refractory to filarial transmission is described. A requirement for success of the strategy is identification of a flight muscle-specific promoter that functions in the mosquito. A GFP marker gene under the control of the promoter region of the Drosophila melanogaster act88F gene was inserted into the genome of Culex quinquefasciatus. Transformation was confirmed by Mendelian genetics. Hybridization of a genomic Southern blot to a radiolabeled probe verified that the entire donor plasmid integrated into the mosquito genome. GFP expression in the transgenic mosquitoes was restricted to the flight muscles.
本文描述了一种构建对丝虫传播具有抗性的库蚊品系的策略。该策略成功的一个必要条件是鉴定出在蚊子中起作用的飞行肌特异性启动子。将受黑腹果蝇act88F基因启动子区域控制的绿色荧光蛋白（GFP）标记基因插入致倦库蚊的基因组中。通过孟德尔遗传学确认了转化。基因组Southern印迹与放射性标记探针的杂交证实整个供体质粒已整合到蚊子基因组中。转基因蚊子中的GFP表达仅限于飞行肌。
