Bittkau Anke, Geyer Roland, Bhatt Manish, Schlosser Dietmar
Department Groundwater Microbiology, UFZ Centre for Environmental Research Leipzig-Halle, Theodor-Lieser-Strasse 4, D-06120 Halle/Saale, Germany.
Toxicology. 2004 Dec 15;205(3):201-10. doi: 10.1016/j.tox.2004.06.052.
Groundwater (GW) from the Bitterfeld industrial region, Central Germany, is contaminated mainly with monochlorobenzene (MCB). Accordingly, current research addresses the development of feasible in situ groundwater remediation technologies. Although easily degradable under aerobic conditions, MCB persists in the essentially anaerobic Bitterfeld aquifer. Therefore, we focused on primary oxidation of MCB and the subsequent anaerobic biodegradability of MCB oxidation products by the indigenous microbial community. In groundwater microcosms, most efficient MCB removal was observed upon treatment with Fenton's reagent (H2O2 + Fe2+), which produces the highly reactive hydroxyl radical and Fe3+ simultaneously. Phospholipid fatty acid analysis following different treatments suggested respective shifts of the microbial community compositions, and indicated that Fenton's reagent had a rather beneficial than an adverse effect on biomass development. Potential metabolites of hydroxyl radical attack on MCB such as chlorohydroquinone, hydroquinone, catechol, resorcinol, and phenol were anaerobically degraded by the groundwater microbial community under Fe3+ -reducing conditions.
德国中部比特费尔德工业区的地下水主要受到一氯苯（MCB）的污染。因此，当前的研究致力于开发可行的原位地下水修复技术。尽管MCB在有氧条件下易于降解，但在本质上厌氧的比特费尔德含水层中仍会持续存在。因此，我们重点研究了MCB的初级氧化以及随后原生微生物群落对MCB氧化产物的厌氧生物降解能力。在地下水微观世界中，用芬顿试剂（H2O2 + Fe2+）处理时观察到了最有效的MCB去除效果，该试剂能同时产生高活性的羟基自由基和Fe3+。不同处理后的磷脂脂肪酸分析表明微生物群落组成发生了相应变化，并表明芬顿试剂对生物量发展具有相当有益而非不利的影响。在Fe3+还原条件下，地下水微生物群落能对羟基自由基攻击MCB产生的潜在代谢产物如氯氢醌、对苯二酚、邻苯二酚、间苯二酚和苯酚进行厌氧降解。
