从泰国家禽群体疫情中分离出的H5N1甲型禽流感病毒的基因组序列分析。
The genome sequence analysis of H5N1 avian influenza A virus isolated from the outbreak among poultry populations in Thailand.
作者信息
Viseshakul Nareerat, Thanawongnuwech Roongroje, Amonsin Alongkorn, Suradhat Sanipa, Payungporn Sunchai, Keawchareon Juthatip, Oraveerakul Kanisak, Wongyanin Piya, Plitkul Sukanya, Theamboonlers Apiradee, Poovorawan Yong
机构信息
Faculty of Veterinary Science, Chulalongkorn University, Pathumwan, Bangkok 10330, Thailand.
出版信息
Virology. 2004 Oct 25;328(2):169-76. doi: 10.1016/j.virol.2004.06.045.
In this report, the genome of the Thai avian influenza virus A (H5N1); A/Chicken/Nakorn-Pathom/Thailand/CU-K2/04, isolated from the Thai avian influenza A (AI) epidemic during the early of 2004 was sequenced. Phylogenetic analyses were performed in comparison to AI viruses from Hong Kong 1997 outbreaks and other AI (H5N1) isolates reported during 2001-2004. Molecular characterization of the Thai AI (H5N1) HA gene revealed a common characteristic of a highly pathogenic AI (HPAI), a 20-codon deletion in the neuraminidase gene, a 5-codon deletion in the NS gene and polymorphisms of the M2 and PB2 genes. Moreover, the HA and NA genes of the Thai AI displayed high similarity to those of the AI viruses isolated from human cases during the same epidemic. Finally, our results demonstrated that the Thai AI emerged as a member of 2000's AI lineage with most of the genetic sequences closely related to the Influenza A/Duck/China/E319.2/03 (H5N1).
在本报告中，对2004年初从泰国禽流感疫情中分离出的泰国甲型禽流感病毒A（H5N1）；A/Chicken/Nakorn-Pathom/Thailand/CU-K2/04的基因组进行了测序。与1997年香港禽流感疫情中的病毒以及2001年至2004年期间报告的其他甲型禽流感（H5N1）分离株进行了系统发育分析。泰国甲型禽流感（H5N1）血凝素（HA）基因的分子特征显示出高致病性禽流感（HPAI）的一个共同特征，即神经氨酸酶基因中有20个密码子缺失，NS基因中有5个密码子缺失，以及M2和PB2基因的多态性。此外，泰国禽流感的HA和NA基因与同一疫情期间从人类病例中分离出的禽流感病毒的HA和NA基因高度相似。最后，我们的结果表明，泰国禽流感作为21世纪禽流感谱系的一员出现，其大部分基因序列与甲型流感病毒/鸭/中国/E319.2/03（H5N1）密切相关。
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