Lim S, Notley-McRobb L, Lim M, Carter D A
School of Molecular and Microbial Biosciences, University of Sydney, Sydney, Australia.
Fungal Genet Biol. 2004 Nov;41(11):1025-36. doi: 10.1016/j.fgb.2004.08.004.
An overview of the character of microsatellites in 14 fungal genomes was obtained by analyzing databases containing complete or nearly complete genome sequences. Low GC content, rather than genome size, was the best predictor of high microsatellite density, although very long iterations of tandem repeats were less common in small genomes. Motif type correlated with %GC in that low-GC genomes were more likely to be dominated by A/T-rich motifs, and vice versa, although some exceptions were noted. The experimentally useful dinucleotide and trinucleotide arrays were analyzed in greater detail. Although these varied in sequence and length among fungal species, some that are likely to be universally useful were identified. This information will be useful for researchers wanting to identify the most useful microsatellites to analyze for the fungi included in this survey and provides a platform for choosing microsatellites to target in fungi that are not yet sequenced.
通过分析包含完整或近乎完整基因组序列的数据库，获得了14个真菌基因组中微卫星特征的概述。低GC含量而非基因组大小是高微卫星密度的最佳预测指标，尽管在小基因组中非常长的串联重复迭代不太常见。基序类型与GC%相关，即低GC基因组更可能由富含A/T的基序主导，反之亦然，不过也注意到了一些例外情况。对实验中有用的二核苷酸和三核苷酸阵列进行了更详细的分析。尽管这些在真菌物种之间的序列和长度各不相同，但还是鉴定出了一些可能普遍有用的阵列。这些信息将有助于研究人员识别对本次调查中所包含的真菌进行分析时最有用的微卫星，并为选择尚未测序的真菌中的微卫星作为目标提供一个平台。
