Dutech Cyril, Enjalbert Jérome, Fournier Elisabeth, Delmotte François, Barrès Benoit, Carlier Jean, Tharreau Didier, Giraud Tatiana
INRA, Biodiversité, Gènes et Communautés, 33612, Cestas, France.
Fungal Genet Biol. 2007 Oct;44(10):933-49. doi: 10.1016/j.fgb.2007.05.003. Epub 2007 Jun 2.
Although they represent powerful genetic markers in many fields of biology, microsatellites have been isolated in few fungal species. The aim of this study was to assess whether obtaining microsatellite markers with an acceptable level of polymorphism is generally harder from fungi than in other organisms. We therefore surveyed the number, nature and polymorphism level of published microsatellite markers in fungi from the literature and from our own data on seventeen fungal microsatellite-enriched libraries, and in five other phylogroups (angiosperms, insects, fishes, birds and mammals). Fungal microsatellites indeed appeared both harder to isolate and to exhibit lower polymorphism than in other organisms. This appeared to be due, at least in part, to genomic specificities, such as scarcity and shortness of fungal microsatellite loci. A correlation was observed between mean repeat number and mean allele number in the published fungal microsatellite loci. The cross-species transferability of fungal microsatellites also appeared lower than in other phylogroups. However, microsatellites have been useful in some fungal species. Thus, the considerable advantages of these markers make their development worthwhile, and this study provides some guidelines for their isolation.
尽管微卫星在许多生物学领域都是强大的遗传标记，但仅在少数真菌物种中分离得到了微卫星。本研究的目的是评估从真菌中获得具有可接受多态性水平的微卫星标记是否通常比在其他生物中更困难。因此，我们从文献以及我们自己关于17个富含微卫星的真菌文库的数据中，调查了已发表的真菌微卫星标记的数量、性质和多态性水平，并与其他五个分类群（被子植物、昆虫、鱼类、鸟类和哺乳动物）进行了比较。与其他生物相比，真菌微卫星确实更难分离，且多态性更低。这似乎至少部分归因于基因组特异性，例如真菌微卫星位点的稀缺性和短小性。在已发表的真菌微卫星位点中，观察到平均重复数与平均等位基因数之间存在相关性。真菌微卫星的种间转移性似乎也低于其他分类群。然而，微卫星在一些真菌物种中已证明是有用的。因此，这些标记的显著优势使其开发具有价值，本研究为其分离提供了一些指导方针。
