Bolzán A D, Lacunza E, Bianchi M S
Laboratorio de Citogenética y Mutagénesis, Instituto Multidisciplinario de Biología Celular (IMBICE), C.C. 403, 1900 La Plata, Buenos Aires, Argentina.
Toxicol In Vitro. 2004 Dec;18(6):901-5. doi: 10.1016/j.tiv.2004.02.002.
We assessed the effect of recombinant IFN-alpha-2a (rIFN-alpha-2a) on the induction of CAs and sister-chromatid exchanges (SCEs) by the methylating compound streptozotocin (STZ), in Chinese Hamster Ovary (CHO) cells. The cytokine was added to cell cultures 30 min before STZ and left in the culture medium until the end of the treatment. A statistically significant increase in the frequency of CAs and SCEs was observed following treatment with STZ alone (p < 0.05) compared to control, whereas treatments with rIFN-alpha-2a alone did not produce any significant increase of CAs or SCEs over the control values (p < 0.05). Moreover, rIFN-alpha-2a had a marked inhibitory effect on the frequency of STZ-induced CAs--both chromosome- and chromatid-type--(p < 0.05) but was unable to prevent SCEs induced by the antibiotic (p > 0.05). A decrease in the replication index (RI) was observed in the combined treatments compared with STZ alone-treated cultures, indicating inhibition of DNA synthesis. It is suggested that rIFN-alpha-2a exerts its protective action against the induction of CAs by STZ by stimulating DNA repair.
我们评估了重组干扰素α-2a（rIFN-α-2a）对甲基化化合物链脲佐菌素（STZ）在中国仓鼠卵巢（CHO）细胞中诱导染色体畸变（CAs）和姐妹染色单体交换（SCEs）的影响。在加入STZ前30分钟将细胞因子添加到细胞培养物中，并保留在培养基中直至处理结束。与对照组相比，单独用STZ处理后观察到CAs和SCEs频率有统计学意义的增加（p<0.05），而单独用rIFN-α-2a处理未使CAs或SCEs频率相对于对照值有任何显著增加（p<0.05）。此外，rIFN-α-2a对STZ诱导的CAs频率——包括染色体型和染色单体型——有显著抑制作用（p<0.05），但无法阻止抗生素诱导的SCEs（p>0.05）。与单独用STZ处理的培养物相比，联合处理组观察到复制指数（RI）降低，表明DNA合成受到抑制。提示rIFN-α-2a通过刺激DNA修复对STZ诱导的CAs发挥保护作用。
