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[尿液试纸条检测对确定尿液中白细胞和细菌的实用性]

[Usefulness of dipstick test for determining leukocytes and bacteria in urine].

作者信息

Takagi S, Arakawa S, Matsumoto O, Kamidono S, Terasoma K, Mita T

机构信息

Department of Urology, Kobe University, School of Medicine.

出版信息

Hinyokika Kiyo. 1992 Jan;38(1):31-6.

PMID:1546566
Abstract

The value of a simple dipstick test (BMtestLN, Boehringer Mannheim-Shionogi) for determination of leukocytes and bacteria in urine was examined. This test consisted of detection of esterase, an enzyme present in leukocytes and the nitrate reduced by bacteria. A total of 666 clinical urine specimens were tested with both tests. The evaluation of leukocytes in urine was compared with microscopic examination, and the identification and measurement of strains was performed using a semiquantitative plate culture method as a reference. The leukocyte esterase test had a sensitivity of 85.3%, a specificity of 67.3%, positive predictive value of 54.7% and negative predictive value of 90.8%. The range of the sensitivity value of nitrate test was 25.7% for the detection of bacteriuria (bacteria greater than or equal to 10(4) CFU/ml). This test had a specificity of 99.6%, positive predictive value of 94.8% and negative value of 82.9%. We concluded that this dipstick test is not useful for determination of the slight change of pyuria, but sensitive for the determination of acute and untreated urinary tract infections.

摘要

对一种用于检测尿液中白细胞和细菌的简易试纸条检测法(BMtestLN,宝灵曼-盐野义)的价值进行了研究。该检测法包括检测酯酶(一种存在于白细胞中的酶)以及被细菌还原的硝酸盐。总共 666 份临床尿液标本用这两种检测法进行了检测。将尿液中白细胞的检测结果与显微镜检查结果进行比较,并以半定量平板培养法作为参考进行菌株的鉴定和计数。白细胞酯酶检测的灵敏度为 85.3%,特异性为 67.3%,阳性预测值为 54.7%,阴性预测值为 90.8%。硝酸盐检测法检测菌尿(细菌数大于或等于 10⁴CFU/ml)的灵敏度范围为 25.7%。该检测法的特异性为 99.6%,阳性预测值为 94.8%,阴性预测值为 82.9%。我们得出结论,这种试纸条检测法对于检测轻度脓尿变化并无用处,但对检测急性和未经治疗的尿路感染很敏感。

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