大肠杆菌O157在牛粪堆中的分布及其对粪便排菌率估计的影响。
Distribution of Escherichia coli O157 in bovine fecal pats and its impact on estimates of the prevalence of fecal shedding.
作者信息
Pearce M C, Fenlon D, Low J C, Smith A W, Knight H I, Evans J, Foster G, Synge B A, Gunn G J
机构信息
Centre for Tropical Veterinary Medicine, University of Edinburgh, Midlothian, UK.
出版信息
Appl Environ Microbiol. 2004 Oct;70(10):5737-43. doi: 10.1128/AEM.70.10.5737-5743.2004.
Abstract
The distribution of Escherichia coli O157 in bovine feces was examined by testing multiple samples from fecal pats and determining the density of E. coli O157 in immunomagnetic separation (IMS)-positive fecal samples. The density of E. coli O157 in bovine feces was highly variable, differing by as much as 76,800 CFU g(-1) between samples from the same fecal pat. The density in most positive samples was <100 CFU g(-1), the limit of reliable detection by IMS. Testing only one 1-g sample of feces per pat with IMS may result in a sensitivity of detection as low as 20 to 50%. It is therefore probable that most surveys have greatly underestimated the prevalence of E. coli O157 shedding in cattle and the proportion of farms with shedding cattle. The sensitivity of the detection of E. coli O157 in bovine feces can be as much as doubled by testing two 1-g samples per pat rather than one 1-g sample.
摘要
通过对粪便堆中的多个样本进行检测，并测定免疫磁珠分离（IMS）阳性粪便样本中大肠杆菌O157的密度，来研究大肠杆菌O157在牛粪中的分布情况。牛粪中大肠杆菌O157的密度变化很大，同一粪便堆中的样本之间相差高达76,800 CFU g(-1)。大多数阳性样本中的密度<100 CFU g(-1)，这是IMS可靠检测的下限。每次粪便堆仅用IMS检测1克粪便样本，可能导致检测灵敏度低至20%至50%。因此，大多数调查很可能大大低估了牛群中大肠杆菌O157的排泄率以及有排泄牛的农场比例。通过每次粪便堆检测两个1克样本而非一个1克样本，牛粪中大肠杆菌O157检测的灵敏度可提高一倍之多。
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