Sano Emiko, Carlson Suzanne, Wegley Linda, Rohwer Forest
Center for Microbial Sciences, San Diego State University, 5500 Campanile Dr., San Diego, CA 92182, USA.
Appl Environ Microbiol. 2004 Oct;70(10):5842-6. doi: 10.1128/AEM.70.10.5842-5846.2004.
Viruses are abundant in all known ecosystems. In the present study, we tested the possibility that viruses from one biome can successfully propagate in another. Viral concentrates were prepared from different near-shore marine sites, lake water, marine sediments, and soil. The concentrates were added to microcosms containing dissolved organic matter as a food source (after filtration to allow 100-kDa particles to pass through) and a 3% (vol/vol) microbial inoculum from a marine water sample (after filtration through a 0.45-microm-pore-size filter). Virus-like particle abundances were then monitored using direct counting. Viral populations from lake water, marine sediments, and soil were able to replicate when they were incubated with the marine microbes, showing that viruses can move between different ecosystems and propagate. These results imply that viruses can laterally transfer DNA between microbes in different biomes.
病毒在所有已知生态系统中都很丰富。在本研究中，我们测试了来自一个生物群落的病毒能否在另一个生物群落中成功繁殖的可能性。病毒浓缩液取自不同的近岸海洋地点、湖水、海洋沉积物和土壤。将浓缩液添加到含有溶解有机物作为食物来源（经过过滤以允许100 kDa的颗粒通过）和来自海水样本的3%（体积/体积）微生物接种物（经过0.45微米孔径过滤器过滤）的微观世界中。然后使用直接计数法监测类病毒颗粒的丰度。当湖水、海洋沉积物和土壤中的病毒种群与海洋微生物一起培养时，它们能够复制，这表明病毒可以在不同生态系统之间移动并繁殖。这些结果意味着病毒可以在不同生物群落的微生物之间横向转移DNA。
