Graduate School of Science and Technology, Kobe University, Rokkodai, Nada-ku, Kobe 657, and Ocean Research Institute, University of Tokyo, Nakano, Tokyo 164, Japan.
Appl Environ Microbiol. 1991 Sep;57(9):2731-4. doi: 10.1128/aem.57.9.2731-2734.1991.
Abundance of bacteria and tiny DNA-associated particles in the upper layer of Japanese coastal and offshore waters was evaluated by epifluorescence microscopy with 0.015-mum-pore-size Nuclepore filters. The number of tiny DNA-associated particles was compared with the abundance of virus particles estimated by transmission electron microscopy. Although a large variation in virus abundance (1.2 x 10 to 35 x 10 ml) was obtained with the transmission electron microscopy method, the ratio of 4',6-diamidino-2-phenylindole-reactive tiny particles to viruses was in a rather narrow range (1.0 to 1.6), indicating that the majority of the tiny DNA-associated particles identified by epifluorescence microscopy were actually virus particles. This result implies the possibility of using epifluorescence microscopy for the evaluation of virus abundance in marine environments.
用带有 0.015 微米孔径的 Nuclepore 过滤器的荧光显微镜评估了日本沿海和近海水域上层的细菌和与微小 DNA 相关的颗粒的丰度。将微小 DNA 相关颗粒的数量与通过透射电子显微镜估计的病毒颗粒的丰度进行了比较。尽管用透射电子显微镜方法获得的病毒丰度（1.2×10 到 35×10 毫升）变化很大，但 4'，6-二脒基-2-苯基吲哚反应性微小颗粒与病毒的比值在一个相当窄的范围内（1.0 到 1.6），表明用荧光显微镜鉴定的大多数与微小 DNA 相关的颗粒实际上是病毒颗粒。这一结果表明，荧光显微镜可能用于评估海洋环境中的病毒丰度。
