Iwai K, Hachisuga T, Iwasaka T, Sugimori H
Department of Obstetrics and Gynecology, Saga Medical School, Japan.
J Obstet Gynaecol (Tokyo 1995). 1995 Dec;21(6):593-600. doi: 10.1111/j.1447-0756.1995.tb00918.x.
In the past, immunohistochemical detection of p53 has been possible only in the case of fresh-frozen tissues. However using CM1 and an enhanced method based on microwave heating and protease digestion enabled the immunohistochemical detection of p53 overexpression in formalin-fixed, paraffin-embedded sections of endometrial carcinoma, although very few positive stainings were obtained without such an enhanced treatment. To evaluate the enhancement method, a comparative study was performed on frozen materials and paraffin-embedded samples.
Fifty-three frozen materials, including normal endometrial glands, endometrial hyperplasias and carcinomas, and paraffin-embedded samples from the same patients were used in this study. PAb1801 (mouse monoclonal antibody) was used to analyse p53 overexpression in the frozen sections, while CM1 (rabbit polyclonal anti-p53 antibody) was used for the paraffin sections.
PAb1801 and CM1 staining on normal endometriums and endometrial lesions showed a significant degree of concordance between frozen and paraffin-embedded materials.
Enhanced immunodetection of p53 in paraffin-embedded tissues will provide a useful alternative to the usual fresh-tissue assay.
过去，p53的免疫组化检测仅适用于新鲜冷冻组织。然而，使用CM1以及基于微波加热和蛋白酶消化的增强方法，能够在福尔马林固定、石蜡包埋的子宫内膜癌切片中进行p53过表达的免疫组化检测，不过在没有这种增强处理的情况下，只能获得极少的阳性染色。为了评估这种增强方法，对冷冻材料和石蜡包埋样本进行了一项对比研究。
本研究使用了53份冷冻材料，包括正常子宫内膜腺体、子宫内膜增生和癌组织，以及来自同一患者的石蜡包埋样本。PAb1801（小鼠单克隆抗体）用于分析冷冻切片中的p53过表达，而CM1（兔多克隆抗p53抗体）用于石蜡切片。
PAb1801和CM1对正常子宫内膜和子宫内膜病变的染色显示，冷冻材料和石蜡包埋材料之间存在显著的一致性。
石蜡包埋组织中p53的增强免疫检测将为常用的新鲜组织检测提供一种有用的替代方法。
